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Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling
The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant e arlier f l owering1 , el1 , which is deficient in a casein kinase I that has critical roles in both plants and animals. el1 had...
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| Published in: | The EMBO journal 2010-06, Vol.29 (11), p.1916-1927 |
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| container_end_page | 1927 |
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| creator | Dai, Cheng Xue, Hong-Wei |
| description | The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant
e
arlier f
l
owering1
,
el1
, which is deficient in a casein kinase I that has critical roles in both plants and animals.
el1
had an enhanced GA response, consistent with the suppression of
EL1
expression by exogenous GA
3
. Biochemical characterization showed that EL1 specifically phosphorylates the rice DELLA protein SLR1, proving a direct evidence for SLR1 phosphorylation. Overexpression of SLR1 in wild‐type plants caused a severe dwarf phenotype, which was significantly suppressed by
EL1
deficiency, indicating the negative effect of SLR1 on GA signalling requires the EL1 function. Further studies showed that the phosphorylation of SLR1 is important for maintaining its activity and stability, and mutation of the candidate phosphorylation site of SLR1 results in the altered GA signalling. This study shows EL1 a novel and key regulator of the GA response and provided important clues on casein kinase I activities in GA signalling and plant development. |
| doi_str_mv | 10.1038/emboj.2010.75 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2885930</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>746083146</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5685-23f36dfb84d0859d551a6a44d8a60a7ee2b6f1809f3ee084d6e26e3fdaa549e83</originalsourceid><addsrcrecordid>eNqFkktv1DAUhSMEokNhyRZZbNg0gx3HjrNBKmE6FFKQyqMSG8tJblJPM3Gwk5b59zidYXhIwMLytfz53MdxEDwmeE4wFc9hXZjVPML-nLA7wYzEHIcRTtjdYIYjTsKYiPQgeODcCmPMRELuBwcRjjFOqZgF63NdAgJl2w2qW3MDVncNOUIKZW9Pj1B_aZxfdtOqARx6tcjzY9RbM4Du0If8nKDBoA4aNehr8BIWmnFCUaOLAiy0reecbjo1Rc3D4F6tWgePdvth8Olk8TF7Hebvl6fZcR6WjAsWRrSmvKoLEVdYsLRijCiu4rgSimOVAEQFr4nAaU0BsKc4RBxoXSnF4hQEPQxebHX7sVhDVUI3WNXK3uq1shtplJa_33T6UjbmWkbC56PYCzzbCVjzdQQ3yLV2pW9HdWBGJxM_ZUH9rP9PUkoiRgX15NM_yJUZrZ-Mk5TxlJNERB4Kt1BpjXMW6n3RBMvJcHlruJwMlwnz_JNfO93TPxz2ANsCN7qFzb_V5OLs5ZspvhWeb9-5fvoSYH9W-7dKdpVrN8C3fSJlryRPqBe_eLeUy-zkM_lylskL-h1amNck</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>356961782</pqid></control><display><type>article</type><title>Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling</title><source>PubMed (Medline)</source><creator>Dai, Cheng ; Xue, Hong-Wei</creator><creatorcontrib>Dai, Cheng ; Xue, Hong-Wei</creatorcontrib><description>The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant
e
arlier f
l
owering1
,
el1
, which is deficient in a casein kinase I that has critical roles in both plants and animals.
el1
had an enhanced GA response, consistent with the suppression of
EL1
expression by exogenous GA
3
. Biochemical characterization showed that EL1 specifically phosphorylates the rice DELLA protein SLR1, proving a direct evidence for SLR1 phosphorylation. Overexpression of SLR1 in wild‐type plants caused a severe dwarf phenotype, which was significantly suppressed by
EL1
deficiency, indicating the negative effect of SLR1 on GA signalling requires the EL1 function. Further studies showed that the phosphorylation of SLR1 is important for maintaining its activity and stability, and mutation of the candidate phosphorylation site of SLR1 results in the altered GA signalling. This study shows EL1 a novel and key regulator of the GA response and provided important clues on casein kinase I activities in GA signalling and plant development.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1038/emboj.2010.75</identifier><identifier>PMID: 20400938</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Botany ; casein kinase I ; EL1 ; EMBO13 ; EMBO30 ; flowering time ; GA response ; Gibberellins - genetics ; Gibberellins - metabolism ; Gibberellins - pharmacology ; Hormones ; Molecular biology ; Mutation ; Oryza - genetics ; Oryza - growth & development ; Oryza - metabolism ; Oryza sativa ; Phosphorylation ; Plant growth ; Plant Growth Regulators - genetics ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Proteins - pharmacology ; Rice ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signal Transduction - physiology</subject><ispartof>The EMBO journal, 2010-06, Vol.29 (11), p.1916-1927</ispartof><rights>European Molecular Biology Organization 2010</rights><rights>Copyright © 2010 European Molecular Biology Organization</rights><rights>Copyright Nature Publishing Group Jun 2, 2010</rights><rights>Copyright © 2010, European Molecular Biology Organization 2010 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5685-23f36dfb84d0859d551a6a44d8a60a7ee2b6f1809f3ee084d6e26e3fdaa549e83</citedby><cites>FETCH-LOGICAL-c5685-23f36dfb84d0859d551a6a44d8a60a7ee2b6f1809f3ee084d6e26e3fdaa549e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885930/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885930/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20400938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Cheng</creatorcontrib><creatorcontrib>Xue, Hong-Wei</creatorcontrib><title>Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant
e
arlier f
l
owering1
,
el1
, which is deficient in a casein kinase I that has critical roles in both plants and animals.
el1
had an enhanced GA response, consistent with the suppression of
EL1
expression by exogenous GA
3
. Biochemical characterization showed that EL1 specifically phosphorylates the rice DELLA protein SLR1, proving a direct evidence for SLR1 phosphorylation. Overexpression of SLR1 in wild‐type plants caused a severe dwarf phenotype, which was significantly suppressed by
EL1
deficiency, indicating the negative effect of SLR1 on GA signalling requires the EL1 function. Further studies showed that the phosphorylation of SLR1 is important for maintaining its activity and stability, and mutation of the candidate phosphorylation site of SLR1 results in the altered GA signalling. This study shows EL1 a novel and key regulator of the GA response and provided important clues on casein kinase I activities in GA signalling and plant development.</description><subject>Botany</subject><subject>casein kinase I</subject><subject>EL1</subject><subject>EMBO13</subject><subject>EMBO30</subject><subject>flowering time</subject><subject>GA response</subject><subject>Gibberellins - genetics</subject><subject>Gibberellins - metabolism</subject><subject>Gibberellins - pharmacology</subject><subject>Hormones</subject><subject>Molecular biology</subject><subject>Mutation</subject><subject>Oryza - genetics</subject><subject>Oryza - growth & development</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>Phosphorylation</subject><subject>Plant growth</subject><subject>Plant Growth Regulators - genetics</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Proteins - pharmacology</subject><subject>Rice</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - physiology</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqFkktv1DAUhSMEokNhyRZZbNg0gx3HjrNBKmE6FFKQyqMSG8tJblJPM3Gwk5b59zidYXhIwMLytfz53MdxEDwmeE4wFc9hXZjVPML-nLA7wYzEHIcRTtjdYIYjTsKYiPQgeODcCmPMRELuBwcRjjFOqZgF63NdAgJl2w2qW3MDVncNOUIKZW9Pj1B_aZxfdtOqARx6tcjzY9RbM4Du0If8nKDBoA4aNehr8BIWmnFCUaOLAiy0reecbjo1Rc3D4F6tWgePdvth8Olk8TF7Hebvl6fZcR6WjAsWRrSmvKoLEVdYsLRijCiu4rgSimOVAEQFr4nAaU0BsKc4RBxoXSnF4hQEPQxebHX7sVhDVUI3WNXK3uq1shtplJa_33T6UjbmWkbC56PYCzzbCVjzdQQ3yLV2pW9HdWBGJxM_ZUH9rP9PUkoiRgX15NM_yJUZrZ-Mk5TxlJNERB4Kt1BpjXMW6n3RBMvJcHlruJwMlwnz_JNfO93TPxz2ANsCN7qFzb_V5OLs5ZspvhWeb9-5fvoSYH9W-7dKdpVrN8C3fSJlryRPqBe_eLeUy-zkM_lylskL-h1amNck</recordid><startdate>20100602</startdate><enddate>20100602</enddate><creator>Dai, Cheng</creator><creator>Xue, Hong-Wei</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>Nature Publishing Group</general><scope>BSCLL</scope><scope>C6C</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20100602</creationdate><title>Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling</title><author>Dai, Cheng ; Xue, Hong-Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5685-23f36dfb84d0859d551a6a44d8a60a7ee2b6f1809f3ee084d6e26e3fdaa549e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Botany</topic><topic>casein kinase I</topic><topic>EL1</topic><topic>EMBO13</topic><topic>EMBO30</topic><topic>flowering time</topic><topic>GA response</topic><topic>Gibberellins - genetics</topic><topic>Gibberellins - metabolism</topic><topic>Gibberellins - pharmacology</topic><topic>Hormones</topic><topic>Molecular biology</topic><topic>Mutation</topic><topic>Oryza - genetics</topic><topic>Oryza - growth & development</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>Phosphorylation</topic><topic>Plant growth</topic><topic>Plant Growth Regulators - genetics</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Proteins - pharmacology</topic><topic>Rice</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dai, Cheng</creatorcontrib><creatorcontrib>Xue, Hong-Wei</creatorcontrib><collection>Istex</collection><collection>Springer Nature OA Free Journals</collection><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Cheng</au><au>Xue, Hong-Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>2010-06-02</date><risdate>2010</risdate><volume>29</volume><issue>11</issue><spage>1916</spage><epage>1927</epage><pages>1916-1927</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant
e
arlier f
l
owering1
,
el1
, which is deficient in a casein kinase I that has critical roles in both plants and animals.
el1
had an enhanced GA response, consistent with the suppression of
EL1
expression by exogenous GA
3
. Biochemical characterization showed that EL1 specifically phosphorylates the rice DELLA protein SLR1, proving a direct evidence for SLR1 phosphorylation. Overexpression of SLR1 in wild‐type plants caused a severe dwarf phenotype, which was significantly suppressed by
EL1
deficiency, indicating the negative effect of SLR1 on GA signalling requires the EL1 function. Further studies showed that the phosphorylation of SLR1 is important for maintaining its activity and stability, and mutation of the candidate phosphorylation site of SLR1 results in the altered GA signalling. This study shows EL1 a novel and key regulator of the GA response and provided important clues on casein kinase I activities in GA signalling and plant development.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>20400938</pmid><doi>10.1038/emboj.2010.75</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0261-4189 1460-2075 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2885930 |
| source | PubMed (Medline) |
| subjects | Botany casein kinase I EL1 EMBO13 EMBO30 flowering time GA response Gibberellins - genetics Gibberellins - metabolism Gibberellins - pharmacology Hormones Molecular biology Mutation Oryza - genetics Oryza - growth & development Oryza - metabolism Oryza sativa Phosphorylation Plant growth Plant Growth Regulators - genetics Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plant Proteins - genetics Plant Proteins - metabolism Plant Proteins - pharmacology Rice Signal Transduction - drug effects Signal Transduction - genetics Signal Transduction - physiology |
| title | Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling |
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