Transcriptional target-based expression cloning of immunoregulatory molecules

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning st...

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Bibliographic Details
Published in:Immunologic research 2010-07, Vol.47 (1-3), p.172-178
Main Authors: Lamason, Rebecca L., Lew, Stefanie M., Pomerantz, Joel L.
Format: Article
Language:English
Subjects:
Allergology
Biomedical and Life Sciences
Biomedicine
Cell Line
Cloning
Cloning, Molecular - methods
Cytokines - genetics
Cytokines - metabolism
Deoxyribonucleic acid
DNA
Gene expression
Gene Expression Regulation
Gene Library
Genes, Reporter
HIV-1 - physiology
Humans
Immune system
Immunology
Internal Medicine
Medicine/Public Health
Molecular biology
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
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Summary:Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.
ISSN:0257-277X
1559-0755
DOI:10.1007/s12026-009-8148-z