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Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling

In many tissues, two or more types of stem cells share a niche, and how the stem cells coordinate their self‐renewal and differentiation is poorly understood. In the Drosophila testis, germ line stem cells (GSCs) and somatic cyst progenitor cells (CPCs) contact each other and share a niche (the hub)...

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Published in:Journal of cellular physiology 2010-05, Vol.223 (2), p.500-510
Main Authors: Singh, Shree Ram, Zheng, Zhiyu, Wang, Hong, Oh, Su-Wan, Chen, Xiu, Hou, Steven X.
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creator Singh, Shree Ram
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description In many tissues, two or more types of stem cells share a niche, and how the stem cells coordinate their self‐renewal and differentiation is poorly understood. In the Drosophila testis, germ line stem cells (GSCs) and somatic cyst progenitor cells (CPCs) contact each other and share a niche (the hub). The hub expresses a growth factor unpaired (Upd) that activates the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in GSCs to regulate the stem cell self‐renewal. Here, we demonstrate that the JAK/STAT signaling also regulates CPCs self‐renewal. We also show that a negative regulator, the suppressor of cytokine signaling 36E (SOCS36E), suppresses JAK/STAT signaling in somatic cells, preventing them from out‐competing the GSCs. Furthermore, through selectively manipulating the JAK/STAT signaling level in either CPCs or GSCs, we demonstrate that the somatic JAK/STAT signaling is essential for self‐renewal and maintenance of both CPCs and GSCs. These data suggest that a single JAK/STAT signal from the niche orchestrate the competitive and dependent co‐existence of GSCs and CPCs in the Drosophila testis niche. J. Cell. Physiol. 223: 500–510, 2010. © 2010 Wiley‐Liss, Inc.
doi_str_mv 10.1002/jcp.22073
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subjects Animals
Cell Communication - physiology
Cell Differentiation - physiology
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Gene Expression Regulation, Developmental - physiology
Germ Cells - cytology
Germ Cells - metabolism
Germ-Line Mutation - genetics
Janus Kinases - genetics
Janus Kinases - metabolism
Male
Signal Transduction - physiology
Spermatogenesis - physiology
STAT Transcription Factors - genetics
STAT Transcription Factors - metabolism
Stem Cell Niche - physiology
Stem Cells - cytology
Stem Cells - metabolism
Suppressor of Cytokine Signaling Proteins - genetics
Suppressor of Cytokine Signaling Proteins - metabolism
Testis - cytology
Testis - embryology
Testis - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
title Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling
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