3D nanoscale imaging of the yeast, Schizosaccharomyces pombe, by full-field transmission X-ray microscopy at 5.4 keV

Three-dimensional (3D) nanoscale structures of the fission yeast, Schizosaccharomyces pombe, can be obtained by full-field transmission hard X-ray microscopy with 30 nm resolution using synchrotron radiation sources. Sample preparation is relatively simple and the samples are portable across various...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2010-07, Vol.397 (6), p.2117-2121
Main Authors: Chen, Jie, Yang, Yunhao, Zhang, Xiaobo, Andrews, Joy C, Pianetta, Piero, Guan, Yong, Liu, Gang, Xiong, Ying, Wu, Ziyu, Tian, Yangchao
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Food Science
image analysis
Imaging
Imaging, Three-Dimensional - instrumentation
Imaging, Three-Dimensional - methods
Laboratory Medicine
Mathematical analysis
Microscope and microscopy
Microscopy - instrumentation
Microscopy - methods
Monitoring/Environmental Analysis
Nanocomposites
Nanomaterials
Nanostructure
Organelles - ultrastructure
Original Paper
Schizosaccharomyces - cytology
Schizosaccharomyces pombe
Staining and Labeling
Synchrotrons
Three dimensional
tomography
X-ray microscopy
X-Rays
Yeast
Yeast subcellular structure
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Summary:Three-dimensional (3D) nanoscale structures of the fission yeast, Schizosaccharomyces pombe, can be obtained by full-field transmission hard X-ray microscopy with 30 nm resolution using synchrotron radiation sources. Sample preparation is relatively simple and the samples are portable across various imaging environments, allowing for high-throughput sample screening. The yeast cells were fixed and double-stained with Reynold's lead citrate and uranyl acetate. We performed both absorption contrast and Zernike phase contrast imaging on these cells in order to test this method. The membranes, nucleus, and subcellular organelles of the cells were clearly visualized using absorption contrast mode. The X-ray images of the cells could be used to study the spatial distributions of the organelles in the cells. These results show unique structural information, demonstrating that hard X-ray microscopy is a complementary method for imaging and analyzing biological samples. [graphic removed]
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-010-3617-8