Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production

The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombi...

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Bibliographic Details
Published in:BioMed research international 2010-01, Vol.2010 (2010), p.1-9
Main Authors: Li, Yi-jing, Ma, Guang-peng, Li, Gui-wei, Qiao, Xin-yuan, Ge, Jun-wei, Tang, Li-jie, Liu, Min, Liu, Li-wei
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Antibodies
Antibody Formation - immunology
Bacteria
Biological and medical sciences
Biomedical research
Blotting, Western
Capsid Proteins - immunology
Cell Line
Cell Proliferation
Cellular
Delivery systems
Electrophoresis, Polyacrylamide Gel
Female
Hogs
Immunoassay
Immunoglobulin A - immunology
Immunoglobulin G - immunology
Lactococcus lactis
Lactococcus lactis - metabolism
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Mortality
Neutralization Tests
Pharmacology. Drug treatments
Plasmids
Polymerase Chain Reaction
Porcine rotavirus
Proteins
Recombinant
Rotavirus Infections - immunology
Rotavirus Infections - prevention & control
Rotavirus Vaccines - immunology
Studies
Sus scrofa - virology
Vaccination
Vaccines
Veterinary medicine
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Summary:The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis. BALB/c mice were immunized orally with VP4-expression recombinant L. lactis and cellular, mucosal and systemic humoral immune responses were examined. Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum. The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells. Our findings suggest that oral immunization with VP4-expressing L. lactis induced both specific local and systemic humoral and cellular immune responses in mice.
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2010/708460