Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution

We demonstrate lensfree holographic microscopy on a chip to achieve approximately 0.6 microm spatial resolution corresponding to a numerical aperture of approximately 0.5 over a large field-of-view of approximately 24 mm2. By using partially coherent illumination from a large aperture (approximately...

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Bibliographic Details
Published in:Optics express 2010-05, Vol.18 (11), p.11181-11191
Main Authors: Bishara, Waheb, Su, Ting-Wei, Coskun, Ahmet F, Ozcan, Aydogan
Format: Article
Language:English
Subjects:
Equipment Design
Equipment Failure Analysis
Holography - instrumentation
Image Enhancement - instrumentation
Lenses
Microscopy - instrumentation
Reproducibility of Results
Semiconductors
Sensitivity and Specificity
Signal Processing, Computer-Assisted - instrumentation
Transducers
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Summary:We demonstrate lensfree holographic microscopy on a chip to achieve approximately 0.6 microm spatial resolution corresponding to a numerical aperture of approximately 0.5 over a large field-of-view of approximately 24 mm2. By using partially coherent illumination from a large aperture (approximately 50 microm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.18.011181