Quantitative fluorescence tomography using a combined tri-modality FT/DOT/XCT system

In this work, a first-of-its-kind fully integrated tri-modality system that combines fluorescence, diffuse optical and x-ray tomography (FT/DOT/XCT) into the same setting is presented. The purpose of this system is to perform quantitative fluorescence tomography using multi-modality imaging approach...

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Bibliographic Details
Published in:Optics express 2010-04, Vol.18 (8), p.7835-7850
Main Authors: Lin, Yuting, Barber, William C, Iwanczyk, Jan S, Roeck, Werner, Nalcioglu, Orhan, Gulsen, Gultekin
Format: Article
Language:English
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Summary:In this work, a first-of-its-kind fully integrated tri-modality system that combines fluorescence, diffuse optical and x-ray tomography (FT/DOT/XCT) into the same setting is presented. The purpose of this system is to perform quantitative fluorescence tomography using multi-modality imaging approach. XCT anatomical information is used as structural priori while optical background heterogeneity information obtained by DOT measurements is used as functional priori. The performance of the hybrid system is evaluated using multi-modality phantoms. In particular, we show that a 2.4 mm diameter fluorescence inclusion located in a heterogeneous medium can be localized accurately with the functional a priori information, although the fluorophore concentration is recovered with 70% error. On the other hand, the fluorophore concentration can be accurately recovered within 8% error only when both DOT optical background functional and XCT structural a priori information are utilized to guide and constrain the FT reconstruction algorithm.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.18.007835