New Automated and High-Throughput Quantitative Analysis of Urinary Ketones by Multifiber Exchange-Solid Phase Microextraction Coupled to Fast Gas Chromatography/Negative Chemical-Electron Ionization/Mass Spectrometry

The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of suppo...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2010-01, Vol.2010 (2010), p.1-13
Main Authors: Dugheri, Stefano, Pacenti, Marco, Traldi, Pietro, Degli Esposti, Filippo, Perchiazzi, Nicola, Franchi, Elena, Calamante, Massimo, Kikic, Ireneo, Alessi, Paolo, Bonacchi, Alice, Salvadori, Edoardo, Arcangeli, Giulio, Cupelli, Vincenzo
Format: Article
Language:English
Subjects:
Analytical chemistry
Automated
Automation
Chemical properties
Chemistry, Analytic
Colleges & universities
Data collection
Devices
Extraction (Chemistry)
Gas chromatography
Ionization
Ketones
Mass spectrometry
Metabolic disorders
Methods
Miniaturization
Quantitative
Solvents
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Summary:The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01–5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.
ISSN:1463-9246
2090-8865
1464-5068
2090-8873
DOI:10.1155/2010/972926