Target RNA-Directed Trimming and Tailing of Small Silencing RNAs

• Published in: Science (American Association for the Advancement of Science) 2010-06, Vol.328 (5985), p.1534-1539
• Main Authors: Ameres, Stefan L, Horwich, Michael D, Hung, Jui-Hung, Xu, Jia, Ghildiyal, Megha, Weng, Zhiping, Zamore, Phillip D
• Format: Article
• Language: English
• Subjects: Animals; Argonaute Proteins; Base Pairing; Biological and medical sciences; Cell Line; Cell lines; Drosophila; Drosophila melanogaster - embryology; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Enzymes; Eukaryotic Initiation Factors - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; Green Fluorescent Proteins - genetics; HeLa cells; Humans; Insects; Messenger RNA; Methylation; Methyltransferases - genetics; Methyltransferases - metabolism; MicroRNA; MicroRNAs - chemistry; MicroRNAs - genetics; MicroRNAs - metabolism; Models, Biological; Molecular and cellular biology; Molecular biology; Molecular genetics; Nucleotides; Renovations; Ribonucleic acid; RNA; RNA Caps; RNA Stability; RNA, Complementary; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - chemistry; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; RNA-Induced Silencing Complex - metabolism; Small interfering RNA; Tailings; Transcription. Transcription factor. Splicing. Rna processing
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1187058

In Drosophila, microRNAs (miRNAs) typically guide Argonaute1 to repress messenger RNA (mRNA), whereas small interfering RNAs (siRNAs) guide Argonaute2 to destroy viral and transposon RNA. Unlike siRNAs, miRNAs rarely form extensive numbers of base pairs to the mRNAs they regulate. We find that extensive complementarity between a target RNA and an Argonaute1-bound miRNA triggers miRNA tailing and 3'-to-5' trimming. In flies, Argonaute2-bound small RNAs--but not those bound to Argonaute1--bear a 2'-O-methyl group at their 3' ends. This modification blocks target-directed small RNA remodeling: In flies lacking Hen1, the enzyme that adds the 2'-O-methyl group, Argonaute2-associated siRNAs are tailed and trimmed. Target complementarity also affects small RNA stability in human cells. These results provide an explanation for the partial complementarity between animal miRNAs and their targets.