Interactome analysis of the six cotton 14-3-3s that are preferentially expressed in fibres and involved in cell elongation

• Published in: Journal of experimental botany 2010-07, Vol.61 (12), p.3331-3344
• Main Authors: Zhang, Ze-Ting, Zhou, Ying, Li, Yang, Shao, Su-Qiang, Li, Bing-Ying, Shi, Hai-Yan, Li, Xue-Bao
• Format: Article
• Language: English
• Subjects: 14-3-3; 14-3-3 proteins; 14-3-3 Proteins - genetics; 14-3-3 Proteins - metabolism; Amino Acid Sequence; Biological and medical sciences; Cell Enlargement; Cell growth; Complementary DNA; Cotton; cotton (Gossypium hirsutum); Cotton Fiber; Cotton fibers; DNA, Complementary - genetics; Fiber cells; fibre development; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene Library; Gossypium - cytology; Gossypium - genetics; Gossypium - metabolism; Molecular Sequence Data; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Protein isoforms; Proteins; protein–protein interaction; RESEARCH PAPER; Research Papers; RNA, Plant - genetics; Sequence Alignment; Sequence Analysis, DNA; Two-Hybrid System Techniques; Yeasts
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erq155

Proteins of the 14-3-3 family regulate a divergent set of signalling pathways in all eukaryotic organisms. In this study, several cDNAs encoding 14-3-3 proteins were isolated from a cotton fibre cDNA library. The Gh14-3-3 genes share high sequence homology at the nucleotide level in the coding region and at the amino acid level. Real-time quantitative RT-PCR analysis indicated that the expression of these Gh14-3-3 genes is developmentally regulated in fibres, and reached their peak at the stage of rapid cell elongation of fibre development. Furthermore, overexpression of Gh14-3-3a, Gh14-3-3e, and Gh14-3-3L in fission yeast promoted atypical longitudinal growth of the host cells. Yeast two-hybrid analysis revealed that the interaction between cotton 14-3-3 proteins is isoform selective. Through yeast two-hybrid screening, 38 novel interaction partners of the six 14-3-3 proteins (Gh14-3-3a, Gh14-3-3e, Gh14-3-3f, Gh14-3-3g, Gh14-3-3h, and Gh14-3-3L), which are involved in plant development, metabolism, signalling transduction, and other cellular processes, were identified in cotton fibres. Taking these data together, it is proposed that the Gh14-3-3 proteins may participate in regulation of fibre cell elongation. Thus, the results of this study provide novel insights into the 14-3-3 signalling related to fibre development of cotton.