The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats

Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem‐repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, t...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal 2010-07, Vol.29 (13), p.2135-2146
Main Authors: Guetg, Claudio, Lienemann, Philipp, Sirri, Valentina, Grummt, Ingrid, Hernandez-Verdun, Danièle, Hottiger, Michael O, Fussenegger, Martin, Santoro, Raffaella
Format: Article
Language:English
Subjects:
Animals
Cell Proliferation
Cellular Biology
Chromosomal Proteins, Non-Histone - metabolism
Deoxyribonucleic acid
DNA
DNA, Ribosomal - genetics
EMBO09
EMBO13
Gene Silencing
Genes, rRNA
Genomic Instability
genomic stability
Genomics
heterochromatin
Heterochromatin - metabolism
Life Sciences
Mice
Molecular biology
NIH 3T3 Cells
NoRC
Proteins
rDNA silencing
Ribonucleic acid
RNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53
cites cdi_FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53
container_end_page 2146
container_issue 13
container_start_page 2135
container_title The EMBO journal
container_volume 29
creator Guetg, Claudio
Lienemann, Philipp
Sirri, Valentina
Grummt, Ingrid
Hernandez-Verdun, Danièle
Hottiger, Michael O
Fussenegger, Martin
Santoro, Raffaella
description Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem‐repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF‐1‐interacting protein‐5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5‐mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.
doi_str_mv 10.1038/emboj.2010.17
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2905252</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733642166</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53</originalsourceid><addsrcrecordid>eNqFks1v0zAYxiMEYt3gyBUZLhOHFNvxR3JBKtXYQKWIqQhulpM4jUsSF9sd63-P05QAk4CLP3_P875-_UbREwSnCCbpS9XmZjPFsN_ze9EEEQZjDDm9H00gZigmKM1OolPnNhBCmnL0MDoJOEtxlk0iu6oVWJrrOShMu23ULWhVqaVXDvhwUyuvrClqa1rpdQcqY_uF6YDsSuC8zHWj_R6YCjjdqM4De72cgbXqgkGPFOEsiJXVBbBqq6R3j6IHlWycenycz6JPby5W86t48eHy7Xy2iAuWER6naZbKikNaEqRQSVFRsjzhFWc5KyGSSZFznBPO0pxQTkoSxgShjFFKcJ7T5Cx6Nfhud3l41CET2Yit1a20e2GkFn_edLoWa3MjcAYppjgYvBgM6juyq9lC9GehnpAmlNygwJ4fg1nzbaecF612hWoa2Smzc4JTknJIDq7_IZOEEYwYC-TzO-TG7GwXaiYYxDwJv8wDFA9QYY1zVlVjpgiKvkPEoUNE3yEC9fzT36sy0j9bIgB0AL6HD93_201cvH_9rl8fjKeDzgVJt1b2V7Z_y-TZIOik31k1RjpQo-nxddp5dTsi0n4VjCecis_LS0H54svHVToPpfsBa23xuA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>602732077</pqid></control><display><type>article</type><title>The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats</title><source>Open Access: PubMed Central</source><creator>Guetg, Claudio ; Lienemann, Philipp ; Sirri, Valentina ; Grummt, Ingrid ; Hernandez-Verdun, Danièle ; Hottiger, Michael O ; Fussenegger, Martin ; Santoro, Raffaella</creator><creatorcontrib>Guetg, Claudio ; Lienemann, Philipp ; Sirri, Valentina ; Grummt, Ingrid ; Hernandez-Verdun, Danièle ; Hottiger, Michael O ; Fussenegger, Martin ; Santoro, Raffaella</creatorcontrib><description>Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem‐repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF‐1‐interacting protein‐5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5‐mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1038/emboj.2010.17</identifier><identifier>PMID: 20168299</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>Animals ; Cell Proliferation ; Cellular Biology ; Chromosomal Proteins, Non-Histone - metabolism ; Deoxyribonucleic acid ; DNA ; DNA, Ribosomal - genetics ; EMBO09 ; EMBO13 ; Gene Silencing ; Genes, rRNA ; Genomic Instability ; genomic stability ; Genomics ; heterochromatin ; Heterochromatin - metabolism ; Life Sciences ; Mice ; Molecular biology ; NIH 3T3 Cells ; NoRC ; Proteins ; rDNA silencing ; Ribonucleic acid ; RNA</subject><ispartof>The EMBO journal, 2010-07, Vol.29 (13), p.2135-2146</ispartof><rights>European Molecular Biology Organization 2010</rights><rights>Copyright © 2010 European Molecular Biology Organization</rights><rights>Copyright Nature Publishing Group Jul 7, 2010</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2010, European Molecular Biology Organization 2010 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53</citedby><cites>FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53</cites><orcidid>0000-0003-0961-8838</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905252/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2905252/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20168299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00505354$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Guetg, Claudio</creatorcontrib><creatorcontrib>Lienemann, Philipp</creatorcontrib><creatorcontrib>Sirri, Valentina</creatorcontrib><creatorcontrib>Grummt, Ingrid</creatorcontrib><creatorcontrib>Hernandez-Verdun, Danièle</creatorcontrib><creatorcontrib>Hottiger, Michael O</creatorcontrib><creatorcontrib>Fussenegger, Martin</creatorcontrib><creatorcontrib>Santoro, Raffaella</creatorcontrib><title>The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem‐repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF‐1‐interacting protein‐5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5‐mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.</description><subject>Animals</subject><subject>Cell Proliferation</subject><subject>Cellular Biology</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Ribosomal - genetics</subject><subject>EMBO09</subject><subject>EMBO13</subject><subject>Gene Silencing</subject><subject>Genes, rRNA</subject><subject>Genomic Instability</subject><subject>genomic stability</subject><subject>Genomics</subject><subject>heterochromatin</subject><subject>Heterochromatin - metabolism</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Molecular biology</subject><subject>NIH 3T3 Cells</subject><subject>NoRC</subject><subject>Proteins</subject><subject>rDNA silencing</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFks1v0zAYxiMEYt3gyBUZLhOHFNvxR3JBKtXYQKWIqQhulpM4jUsSF9sd63-P05QAk4CLP3_P875-_UbREwSnCCbpS9XmZjPFsN_ze9EEEQZjDDm9H00gZigmKM1OolPnNhBCmnL0MDoJOEtxlk0iu6oVWJrrOShMu23ULWhVqaVXDvhwUyuvrClqa1rpdQcqY_uF6YDsSuC8zHWj_R6YCjjdqM4De72cgbXqgkGPFOEsiJXVBbBqq6R3j6IHlWycenycz6JPby5W86t48eHy7Xy2iAuWER6naZbKikNaEqRQSVFRsjzhFWc5KyGSSZFznBPO0pxQTkoSxgShjFFKcJ7T5Cx6Nfhud3l41CET2Yit1a20e2GkFn_edLoWa3MjcAYppjgYvBgM6juyq9lC9GehnpAmlNygwJ4fg1nzbaecF612hWoa2Smzc4JTknJIDq7_IZOEEYwYC-TzO-TG7GwXaiYYxDwJv8wDFA9QYY1zVlVjpgiKvkPEoUNE3yEC9fzT36sy0j9bIgB0AL6HD93_201cvH_9rl8fjKeDzgVJt1b2V7Z_y-TZIOik31k1RjpQo-nxddp5dTsi0n4VjCecis_LS0H54svHVToPpfsBa23xuA</recordid><startdate>20100707</startdate><enddate>20100707</enddate><creator>Guetg, Claudio</creator><creator>Lienemann, Philipp</creator><creator>Sirri, Valentina</creator><creator>Grummt, Ingrid</creator><creator>Hernandez-Verdun, Danièle</creator><creator>Hottiger, Michael O</creator><creator>Fussenegger, Martin</creator><creator>Santoro, Raffaella</creator><general>John Wiley &amp; Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>EMBO Press</general><general>Nature Publishing Group</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0961-8838</orcidid></search><sort><creationdate>20100707</creationdate><title>The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats</title><author>Guetg, Claudio ; Lienemann, Philipp ; Sirri, Valentina ; Grummt, Ingrid ; Hernandez-Verdun, Danièle ; Hottiger, Michael O ; Fussenegger, Martin ; Santoro, Raffaella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell Proliferation</topic><topic>Cellular Biology</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Ribosomal - genetics</topic><topic>EMBO09</topic><topic>EMBO13</topic><topic>Gene Silencing</topic><topic>Genes, rRNA</topic><topic>Genomic Instability</topic><topic>genomic stability</topic><topic>Genomics</topic><topic>heterochromatin</topic><topic>Heterochromatin - metabolism</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Molecular biology</topic><topic>NIH 3T3 Cells</topic><topic>NoRC</topic><topic>Proteins</topic><topic>rDNA silencing</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guetg, Claudio</creatorcontrib><creatorcontrib>Lienemann, Philipp</creatorcontrib><creatorcontrib>Sirri, Valentina</creatorcontrib><creatorcontrib>Grummt, Ingrid</creatorcontrib><creatorcontrib>Hernandez-Verdun, Danièle</creatorcontrib><creatorcontrib>Hottiger, Michael O</creatorcontrib><creatorcontrib>Fussenegger, Martin</creatorcontrib><creatorcontrib>Santoro, Raffaella</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guetg, Claudio</au><au>Lienemann, Philipp</au><au>Sirri, Valentina</au><au>Grummt, Ingrid</au><au>Hernandez-Verdun, Danièle</au><au>Hottiger, Michael O</au><au>Fussenegger, Martin</au><au>Santoro, Raffaella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>2010-07-07</date><risdate>2010</risdate><volume>29</volume><issue>13</issue><spage>2135</spage><epage>2146</epage><pages>2135-2146</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem‐repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF‐1‐interacting protein‐5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5‐mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>20168299</pmid><doi>10.1038/emboj.2010.17</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0961-8838</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0261-4189
ispartof The EMBO journal, 2010-07, Vol.29 (13), p.2135-2146
issn 0261-4189
1460-2075
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2905252
source Open Access: PubMed Central
subjects Animals
Cell Proliferation
Cellular Biology
Chromosomal Proteins, Non-Histone - metabolism
Deoxyribonucleic acid
DNA
DNA, Ribosomal - genetics
EMBO09
EMBO13
Gene Silencing
Genes, rRNA
Genomic Instability
genomic stability
Genomics
heterochromatin
Heterochromatin - metabolism
Life Sciences
Mice
Molecular biology
NIH 3T3 Cells
NoRC
Proteins
rDNA silencing
Ribonucleic acid
RNA
title The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T06%3A33%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20NoRC%20complex%20mediates%20the%20heterochromatin%20formation%20and%20stability%20of%20silent%20rRNA%20genes%20and%20centromeric%20repeats&rft.jtitle=The%20EMBO%20journal&rft.au=Guetg,%20Claudio&rft.date=2010-07-07&rft.volume=29&rft.issue=13&rft.spage=2135&rft.epage=2146&rft.pages=2135-2146&rft.issn=0261-4189&rft.eissn=1460-2075&rft.coden=EMJODG&rft_id=info:doi/10.1038/emboj.2010.17&rft_dat=%3Cproquest_pubme%3E733642166%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c6947-8898af705d41e1d51cd6b37f76b6d01a3cb72b4768b4574d4457311965542bb53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=602732077&rft_id=info:pmid/20168299&rfr_iscdi=true