Vitellogenin gene expression in autogenous Culex tarsalis

Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is...

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Bibliographic Details
Published in:Insect molecular biology 2010-08, Vol.19 (4), p.423-429
Main Authors: Provost-Javier, K.N, Chen, S, Rasgon, J.L
Format: Article
Language:English
Subjects:
Aedes aegypti
Animals
autogeny
Chickens
Culex - genetics
Culex - growth & development
Culex tarsalis
Feeding Behavior
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Larva - genetics
Larva - growth & development
Ovary - cytology
Ovary - metabolism
Pupa - genetics
Pupa - growth & development
Vitellogenesis - genetics
vitellogenin genes
Vitellogenins - genetics
Vitellogenins - metabolism
West Nile virus
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Summary:Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but little is known about regulation of vitellogenesis in autogenous mosquitoes. We characterized the expression of four vitellogenin genes (Vg1a, Vg1b, Vg2a and Vg2b) in an autogenous strain of the West Nile Virus vector, Culex tarsalis. All vitellogenin genes were expressed during autogenous reproduction and following a bloodmeal, although the intensity and duration of expression varied amongst genes. Quantitative PCR analysis of vitellogenin transcription during autogeny revealed a similar temporal pattern to known vitellogenin expression profiles in anautogenous Aedes aegypti. Vitellogenin transcript, primarily produced from the Vg1b gene, was also detected in the larval and pupal stages of development, but no detectable vitellogenin protein was produced during this time period.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2010.00999.x