Clinically Relevant Cell Sources for TMJ Disc Engineering

Tissue-engineering of the temporomandibular joint (TMJ) disc aims to provide patients with TMJ disorders an option to replace diseased tissue with autologous, functional tissue. This study examined clinically relevant cell sources by comparing costal chondrocytes, dermal fibroblasts, a mixture of th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dental research 2008-06, Vol.87 (6), p.548-552
Main Authors: Johns, D.E., Wong, M.E., Athanasiou, K.A.
Format: Article
Language:English
Subjects:
Animals
Arthroplasty, Replacement
Cells, Cultured
Chondrocytes - metabolism
Collagen Type I - biosynthesis
Collagen Type II - biosynthesis
Compressive Strength
Dental Stress Analysis
Dentistry
Elasticity
Extracellular Matrix Proteins - biosynthesis
Female
Fibroblasts - metabolism
Glycosaminoglycans - biosynthesis
Goats
Ribs - cytology
Skin - cytology
Temporomandibular Joint Disc - cytology
Tensile Strength
Tissue Engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tissue-engineering of the temporomandibular joint (TMJ) disc aims to provide patients with TMJ disorders an option to replace diseased tissue with autologous, functional tissue. This study examined clinically relevant cell sources by comparing costal chondrocytes, dermal fibroblasts, a mixture of the two, and TMJ disc cells in a scaffoldless tissue-engineering approach. It was hypothesized that all constructs would produce matrix relevant to the TMJ disc, but the mixture constructs were expected to appear most like the TMJ disc constructs. Costal chondrocyte and mixture constructs were morphologically and biochemically superior to the TMJ disc and dermal fibroblast constructs, and their compressive properties were not significantly different. Costal chondrocyte constructs produced almost 40 times more collagen and 800 times more glycosaminoglycans than did TMJ constructs. This study demonstrates the ability of costal chondrocytes to produce extracellular matrix that may function in a TMJ disc replacement.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910808700609