Substantivity of chlorhexidine to human dentin

• Published in: Dental materials 2010-08, Vol.26 (8), p.779-785
• Main Authors: Carrilho, Marcela R, Carvalho, Ricardo M, Sousa, Ethan N, Nicolau, José, Breschi, Lorenzo, Mazzoni, Annalisa, Tjäderhane, Leo, Tay, Franklin R, Agee, Kelli, Pashley, David H
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Analysis of Variance; Anti-Infective Agents, Local - analysis; Anti-Infective Agents, Local - metabolism; Anti-Infective Agents, Local - pharmacology; Binding; Chlorhexidine; Chlorhexidine - analysis; Chlorhexidine - metabolism; Chlorhexidine - pharmacology; Demineralizing; Dental Bonding; Dentin; Dentin - metabolism; Dentistry; Disks; Distilled water; Durability; Durapatite - metabolism; Human; Humans; Hydroxyapatite; Matrix Metalloproteinase Inhibitors; Phosphoric acid; Protease Inhibitors - pharmacology; Protein Binding; Substantivity; Tooth Demineralization - metabolism
• ISSN: 0109-5641; 1879-0097
• DOI: 10.1016/j.dental.2010.04.002

Abstract Objectives To better comprehend the role of CHX in the preservation of resin–dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods Dentin disks ( n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 μL of distilled water (controls), (2) 10 μL of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 μL of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 °C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation ( p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) ( p < 0.05). Conclusion The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin–dentin bonds.