Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints

Purpose To understand cell cycle controls in the 8-Cell human blastomere. Methods Data from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripotency...

Full description

Saved in:
Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2010-06, Vol.27 (6), p.265-276
Main Authors: Kiessling, Ann A., Bletsa, Ritsa, Desmarais, Bryan, Mara, Christina, Kallianidis, Kostas, Loutradis, Dimitris
Format: Article
Language:English
Subjects:
Blastomeres - cytology
Blastomeres - metabolism
Cell cycle
Cell Cycle - genetics
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell division
Cell Line
Chromosomes
Databases, Genetic
Embryo Biology
Embryonic Development - genetics
Embryos
Fibroblasts
Gene Expression Regulation, Developmental
Gene Library
Genes
Genome, Human
Genomes
Growth factors
Gynecology
HeLa Cells
Human Genetics
Humans
Kinases
Medicine
Medicine & Public Health
Oligonucleotide Array Sequence Analysis
Proteins
Reproductive Medicine
RNA Interference
Stem cells
Transcription factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose To understand cell cycle controls in the 8-Cell human blastomere. Methods Data from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripotency, and embryonic stem cells. A sub database of 3,803 genes identified by high throughput RNA knock-down studies, plus genes that oscillate in human cells, was analyzed. Results Thirty-five genes over-detected at least 7-fold specifically on the 8-Cell arrays were enriched for cell cycle drivers and for proteins that stabilize chromosome cohesion and spindle attachment and limit DNA and centrosome replication to once per cycle. Conclusions These results indicate that 8-cell human blastomere cleavage is guided by cyclic over-expression of key proteins, rather than canonical checkpoints, leading to rapidly increasing gene copy number and a susceptibility to chromosome and cytokinesis mishaps, well-noted characteristics of preimplantation human embryos.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-010-9407-6