Distinct roles for two synaptotagmin isoforms in synchronous and asynchronous transmitter release at zebrafish neuromuscular junction

An obligatory role for the calcium sensor synaptotagmins in stimulus-coupled release of neurotransmitter is well established, but a role for synaptotagmin isoform involvement in asynchronous release remains conjecture. We show, at the zebrafish neuromuscular synapse, that two separate synaptotagmins...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2010-08, Vol.107 (31), p.13906-13911
Main Authors: Wen, Hua, Linhoff, Michael W., McGinley, Matthew J., Li, Geng-Lin, Corson, Glen M., Mandel, Gail, Brehm, Paul
Format: Article
Language:English
Subjects:
Action potentials
Animals
Biological Sciences
Calcium
Danio rerio
Exocytosis
Exons
Freshwater
Molecular Sequence Data
Neuromuscular Junction - metabolism
Neurons
Neurotransmitters
Protein Isoforms - genetics
Protein Isoforms - metabolism
Sensors
Synapses
Synaptic Transmission
Synaptotagmins
Synaptotagmins - genetics
Synaptotagmins - metabolism
Transcription, Genetic
Zebrafish
Zebrafish - metabolism
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Summary:An obligatory role for the calcium sensor synaptotagmins in stimulus-coupled release of neurotransmitter is well established, but a role for synaptotagmin isoform involvement in asynchronous release remains conjecture. We show, at the zebrafish neuromuscular synapse, that two separate synaptotagmins underlie these processes. Specifically, knockdown of synaptotagmin 2 (syt2) reduces synchronous release, whereas knockdown of synaptotagmin 7 (syt7) reduces the asynchronous component of release. The zebrafish neuromuscular junction is unique in having a very small quantal content and a high release probability under conditions of either low-frequency stimulation or high-frequency augmentation. Through these features, we further determined that during the height of shared synchronous and asynchronous transmission these two modes compete for the same release sites.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1008598107