Long Double-Stranded RNA Produces Specific Gene Downregulation in Giardia lamblia

In many eukaryotes, the introduction of double-stranded RNA (dsRNA) into cells triggers the degradation of mRNAs through a post-transcriptional gene-silencing mechanism called RNA interference or RNAi. In the present study, we found that endogenous long-dsRNA was substantially more effective at prod...

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Bibliographic Details
Published in:The Journal of parasitology 2010-08, Vol.96 (4), p.815-819
Main Authors: Rivero, Maria R, Kulakova, Liudmila, Touz, Maria C
Format: Article
Language:English
Subjects:
Adaptin
Adaptor Protein Complex Subunits - genetics
Adaptor Protein Complex Subunits - metabolism
Cloning
Cysts
Double stranded RNA
Down regulation
Down-Regulation - genetics
Fluorescent Antibody Technique, Direct
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation - genetics
Genes
Giardia lamblia
Giardia lamblia - genetics
Giardia lamblia - metabolism
Green Fluorescent Proteins - metabolism
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Messenger RNA
Microscopy, Confocal
Parasites
Protein expression
Protein synthesis
Proteins
Protozoa
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
RESEARCH NOTES
RNA
RNA interference
RNA Interference - physiology
RNA polymerase
RNA Replicase - genetics
RNA Replicase - metabolism
RNA, Double-Stranded - physiology
RNA, Messenger - metabolism
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Trophozoites
Tubulin - genetics
Tubulin - metabolism
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Summary:In many eukaryotes, the introduction of double-stranded RNA (dsRNA) into cells triggers the degradation of mRNAs through a post-transcriptional gene-silencing mechanism called RNA interference or RNAi. In the present study, we found that endogenous long-dsRNA was substantially more effective at producing interference than endogenous, or exogenous, short-dsRNA expression in Giardia lamblia. The effects of this interference were not evident in the highly expressed protein tubulin or the stage-specific cyst wall protein 2. However, long-dsRNA caused potent and specific interference in the medium subunits of adaptins, the RNA-dependent RNA polymerase, and the exogenous green fluorescence protein. Our results suggest that the ability of dsRNA antisense to inhibit the expression of these specific types of proteins is indicative of a gene-specific mechanism.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-2406.1