Detection of increased scyllo-inositol in brain with magnetic resonance spectroscopy after dietary supplementation in Alzheimer’s disease mouse models

• Published in: Neuropharmacology 2010-09, Vol.59 (4), p.353-357
• Main Authors: Choi, Ji-Kyung, Carreras, Isabel, Dedeoglu, Alpaslan, Jenkins, Bruce G.
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - diet therapy; Alzheimer Disease - metabolism; Alzheimer’s disease; Animals; Brain - metabolism; Dietary Supplements; Disease Models, Animal; Female; Humans; Inositol - administration & dosage; Inositol - biosynthesis; Inositol - metabolism; Magic angle spinning; Magnetic Resonance Spectroscopy - methods; Mice; Mice, Transgenic; MRS; Presenilin-1 - genetics; Presenilin-1 - metabolism; Scyllo-inositol; Transgenic mouse
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2010.03.011

There is evidence that inositol isomers may help protect against formation of toxic fibrils of Aβ fragments in Alzheimer's disease mouse models. Scyllo-inositol is one of the more promising inositol isomers for the potential treatment of Alzheimer’s disease (AD) and can be detected using MRS in human subjects. In this manuscript we demonstrate using MRS, in two different mouse models of AD (APP × PS1 and APP × PS1 × tau), that we could detect increased scyllo-inositol in the hippocampus and frontal cortex in mice fed water supplemented with 16.5 mg/L of scyllo-inositol equivalent to about 3.3  mg/kg/day. We used both brain extracts using solution MRS as well as intact brain tissue using high resolution magic angle spinning (HRMAS) to ensure that any membrane-associated scyllo-inositol would be detected. By brain extracts we detected a 3.0 fold increase in scyllo-inositol in the scyllo-fed AD mice compared to normal diet ( p