Potent immune responses and in vitro pro-inflammatory cytokine suppression by a novel adenovirus vaccine vector based on rare human serotype 28

Abstract Adenovirus vaccine vectors derived from rare human serotypes have been shown to be less potent than serotype 5 (Ad5) at inducing immune responses to encoded antigens. To identify highly immunogenic adenovirus vectors, we assessed pro-inflammatory cytokine expression, binding to the CD46 rec...

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Bibliographic Details
Published in:Vaccine 2010-08, Vol.28 (35), p.5691-5702
Main Authors: Kahl, Christoph A, Bonnell, Jessica, Hiriyanna, Suja, Fultz, Megan, Nyberg-Hoffman, Cassandra, Chen, Ping, King, C. Richter, Gall, Jason G.D
Format: Article
Language:English
Subjects:
Adenovirus
Adenoviruses, Human - immunology
Adolescent
Adult
Aged
Allergy and Immunology
Animal vaccines
Animals
Antibody Formation
Applied microbiology
Biological and medical sciences
CD8-Positive T-Lymphocytes - immunology
Cell Line
Cytokines - immunology
Deoxyribonucleic acid
DNA
Female
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Genomes
Human vaccine
Humans
Immune system
Immunity, Cellular
Immunogenicity
Influenza
Leukocytes, Mononuclear - immunology
Lymphocytes
Male
Membrane Cofactor Protein - immunology
Mice
Mice, Inbred BALB C
Microbiology
Middle Aged
Miscellaneous
Neutralization Tests
Proteins
Rodents
Seroepidemiologic Studies
Seroprevalence
Studies
United States
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vector
Viral Vaccines - immunology
Virology
Young Adult
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Summary:Abstract Adenovirus vaccine vectors derived from rare human serotypes have been shown to be less potent than serotype 5 (Ad5) at inducing immune responses to encoded antigens. To identify highly immunogenic adenovirus vectors, we assessed pro-inflammatory cytokine expression, binding to the CD46 receptor, and immunogenicity. Species D adenoviruses uniquely suppressed pro-inflammatory cytokines and induced high levels of type I interferon. Thus, it was unexpected that a vector derived from a representative serotype, Ad28, induced significantly higher transgene-specific T cell responses than an Ad35 vector. Prime–boost regimens with Ad28, Ad35, Ad14, or Ad5 significantly boosted T cell and antibody responses. The seroprevalence of Ad28 was confirmed to be
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2010.06.050