Adipose-Derived Mesenchymal Stem Cells Treated with Growth Differentiation Factor-5 Express Tendon-Specific Markers

Objectives: Adipose-derived mesenchymal stem cells (ADMSCs) are a unique population of stem cells with therapeutic potential in the treatment of connective tissue injuries. Growth differentiation factor-5 (GDF)-5 is known to play a role in tendon repair and maintenance. The aim of this study was to...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part A 2010-09, Vol.16 (9), p.2941-2951
Main Authors: Park, Andrew, Hogan, MaCalus V., Kesturu, Girish S., James, Roshan, Balian, Gary, Chhabra, Abhinav Bobby
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose tissues
Aggrecans - metabolism
Animals
Basic Helix-Loop-Helix Transcription Factors - metabolism
Biomarkers - metabolism
Blotting, Western
Body fat
Cell Differentiation - drug effects
Cell growth
Cell Proliferation - drug effects
Collagen Type I - metabolism
Decorin - metabolism
Gene expression
Genetic aspects
Genetic markers
Growth
Growth Differentiation Factor 5 - pharmacology
Identification and classification
Matrix Metalloproteinase 13 - metabolism
Membrane Proteins - metabolism
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Original
Original Articles
Physiological aspects
Polymerase Chain Reaction
Proteins
Rats
Rats, Inbred F344
Stem cells
Tenascin - metabolism
Tendons
Tendons - metabolism
Tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives: Adipose-derived mesenchymal stem cells (ADMSCs) are a unique population of stem cells with therapeutic potential in the treatment of connective tissue injuries. Growth differentiation factor-5 (GDF)-5 is known to play a role in tendon repair and maintenance. The aim of this study was to investigate the effects of GDF-5 on proliferation and tendonogenic gene expression of rat ADMSCs. Methods: ADMSCs were treated in culture with different concentrations of GDF-5 (0–1000 ng/mL) for 12 days. Biochemical, temporal, and concentration kinetic studies were done. Extracellular matrix (ECM) synthesis, tendonogenic differentiation, and matrix remodeling gene and protein expression were analyzed. Results: GDF-5 led to increased ADMSC proliferation in a dose- and time-dependent manner. ADMSCs demonstrated enhanced ECM ( collagen type I, decorin, and aggrecan ) and tendonogenic marker ( scleraxis, tenomodulin, and tenascin-C ) gene expression with 100 ng/mL of GDF-5 ( p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2009.0710