Detection of Early and Single Infections of Schistosoma japonicum in the Intermediate Host Snail, Oncomelania hupensis, by PCR and Loop-Mediated Isothermal Amplification (LAMP) Assay

Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2010-09, Vol.83 (3), p.542-548
Main Authors: KUMAGAI, Takashi, FURUSHIMA-SHIMOGAWARA, Rieko, OHMAE, Hiroshi, WANG, Tian-Ping, SHAOHONG LU, RUI CHEN, LIYONG WEN, OHTA, Nobuo
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Diseases caused by trematodes
DNA Primers
DNA, Ribosomal - genetics
Helminthic diseases
Infectious diseases
Medical sciences
Mice
Mice, Inbred ICR
Parasitic diseases
Polymerase Chain Reaction - methods
Schistosoma japonicum - genetics
Schistosoma japonicum - isolation & purification
Schistosomiases
Snails - parasitology
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Summary:Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one miracidium at just 1 day after infection. We compared these results with those from loop-mediated isothermal amplification (LAMP) targeting 28S rDNA and found similar results. The LAMP could amplify the specific DNA from a group of 100 normal snails mixed with one infected snail A PCR screening of infected snails from endemic regions in Anhui Province revealed schistosomal DNA even in snails found negative by microscopy. PCR and LAMP show promise for monitoring the early infection rate in snails, and they may be useful for predicting the risk of infection in the endemic places.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2010.10-0016