A Metabonomic Approach to Analyze the Dexamethasone-Induced Cleft Palate in Mice

Mice models are an important way to understand the relation between the fetus with cleft palate and changes of maternal biofluid. This paper aims to develop a metabonomics approach to analyze dexamethasone-induced cleft palate in pregnant C57BL/6J mice and to study the relationship between the chang...

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Bibliographic Details
Published in:BioMed research international 2011-01, Vol.2011 (2011), p.1-8
Main Authors: Zhou, Jinglin, Xu, Bin, Shi, Bing, Huang, Jing, He, Wei, Lu, Shengjun, Lu, Junjun, Xiao, Liying, Li, Wei
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biomarkers - blood
Chi-Square Distribution
Cleft palate
Cleft Palate - blood
Cleft Palate - chemically induced
Cleft Palate - metabolism
Cleft Palate - pathology
Cluster analysis
Development and progression
Dexamethasone
Dexamethasone - toxicity
Discriminant analysis
Female
Fetus - pathology
Fetuses
Histocytochemistry
Laboratory animals
Male
Mathematical models
Metabolites
Metabolomics - methods
Methods
Mice
Mice, Inbred C57BL
Pattern recognition
Physiological aspects
Plasma
Pregnancy
Prenatal Diagnosis
Principal Component Analysis
Principal components analysis
Risk factors
Spectrum analysis
Studies
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Summary:Mice models are an important way to understand the relation between the fetus with cleft palate and changes of maternal biofluid. This paper aims to develop a metabonomics approach to analyze dexamethasone-induced cleft palate in pregnant C57BL/6J mice and to study the relationship between the change of endogenous small molecular metabolites in maternal plasma and the incidence of cleft palate. To do so, pregnant mice were randomly divided into two groups. The one group was injected with dexamethasone. On E17.5th day, the incident rates of cleft palate from embryos in two groups were calculated. The H1-NMR spectra from the metabolites in plasma in two groups was collected at same time. Then the data were analyzed using metabonomics methods (PCA and SIMCA). The results showed that the data from the two groups displayed distinctive characters, and the incidence of cleft palate were significantly different (P
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/509043