Localization Microscopy Reveals Expression-Dependent Parameters of Chromatin Nanostructure

A combined approach of 2D high-resolution localization light microscopy and statistical methods is presented to infer structural features and density fluctuations at the nuclear nanoscale. Hallmarks of nuclear nanostructure are found on the scale below 100 nm for both human fibroblast and HeLa cells...

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Bibliographic Details
Published in:Biophysical journal 2010-09, Vol.99 (5), p.1358-1367
Main Authors: Bohn, Manfred, Diesinger, Philipp, Kaufmann, Rainer, Weiland, Yanina, Müller, Patrick, Gunkel, Manuel, von Ketteler, Alexa, Lemmer, Paul, Hausmann, Michael, Heermann, Dieter W., Cremer, Christoph
Format: Article
Language:English
Subjects:
Biophysics
Cell Nucleus - metabolism
Cells
Cellular Biophysics and Electrophysiology
Chromatin
Chromatin - chemistry
Chromatin - metabolism
Fluorescent Dyes - metabolism
Gene Expression Regulation
HeLa Cells
Histones - chemistry
Histones - metabolism
Humans
Image Processing, Computer-Assisted
Light
Microscopy - methods
Microscopy - statistics & numerical data
Nanostructured materials
Nanostructures
Protein Transport
Proteins
Statistical methods
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Summary:A combined approach of 2D high-resolution localization light microscopy and statistical methods is presented to infer structural features and density fluctuations at the nuclear nanoscale. Hallmarks of nuclear nanostructure are found on the scale below 100 nm for both human fibroblast and HeLa cells. Mechanical measures were extracted as a quantitative tool from the histone density fluctuations inside the cell to obtain structural fluctuations on the scale of several micrometers. Results show that different mechanisms of expression of the same nuclear protein type lead to significantly different patterns on the nanoscale and to pronounced differences in the detected compressibility of chromatin. The observed fluctuations, including the experimental evidence for dynamic looping, are consistent with a recently proposed chromatin model.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2010.05.043