Norwalk virus does not replicate in human macrophages or dendritic cells derived from the peripheral blood of susceptible humans

Abstract Human noroviruses are difficult to study due to the lack of an efficient in vitro cell culture system or small animal model. Murine norovirus replicates in murine macrophages (MΦ) and dendritic cells (DCs), raising the possibility that human NoVs might replicate in such human cell types. To...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2010-10, Vol.406 (1), p.1-11
Main Authors: Lay, Margarita K, Atmar, Robert L, Guix, Susana, Bharadwaj, Uddalak, He, Hong, Neill, Frederick H, Sastry, K. Jagannadha, Yao, Qizhi, Estes, Mary K
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Adult
Animals
Antigens, Viral - metabolism
Base Sequence
Caliciviridae Infections - genetics
Caliciviridae Infections - physiopathology
Caliciviridae Infections - virology
CX3C Chemokine Receptor 1
Dendritic cells
Dendritic Cells - classification
Dendritic Cells - immunology
Dendritic Cells - virology
DNA Primers - genetics
Fucosyltransferases - genetics
Galactoside 2-alpha-L-fucosyltransferase
Genotype
GPI-Linked Proteins
Humans
In Vitro Techniques
Infectious Disease
Interferon Type I - metabolism
Lipopolysaccharide Receptors - metabolism
Macrophages
Macrophages - immunology
Macrophages - virology
Mice
Norovirus
Norwalk virus
Norwalk virus - classification
Norwalk virus - pathogenicity
Norwalk virus - physiology
Receptors, Chemokine - metabolism
Receptors, IgG - metabolism
Secretor-positive donors
Species Specificity
Viral Tropism
Virus Replication
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Summary:Abstract Human noroviruses are difficult to study due to the lack of an efficient in vitro cell culture system or small animal model. Murine norovirus replicates in murine macrophages (MΦ) and dendritic cells (DCs), raising the possibility that human NoVs might replicate in such human cell types. To test this hypothesis, we evaluated DCs and MΦ derived from monocyte subsets and CD11c+ DCs isolated from peripheral blood mononuclear cells of individuals susceptible to Norwalk virus (NV) infection. These cells were exposed to NV and replication was evaluated by immunofluorescence and by quantitative RT-PCR. A few PBMC-derived DCs expressed NV proteins. However, NV RNA did not increase in any of the cells tested. These results demonstrate that NV does not replicate in human CD11c+ DCs, monocyte-derived DCs and MΦ, but abortive infection may occur in a few DCs. These results suggest that NV tropism is distinct from that of murine noroviruses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2010.07.001