Caulobacter PopZ forms a polar subdomain dictating sequential changes in pole composition and function

The bacterium Caulobacter crescentus has morphologically and functionally distinct cell poles that undergo sequential changes during the cell cycle. We show that the PopZ oligomeric network forms polar ribosome exclusion zones that change function during cell cycle progression. The parS/ParB chromos...

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Bibliographic Details
Published in:Molecular microbiology 2010-04, Vol.76 (1), p.173-189
Main Authors: Bowman, Grant R, Comolli, Luis R, Gaietta, Guido M, Fero, Michael, Hong, Sun-Hae, Jones, Ying, Lee, Julie H, Downing, Kenneth H, Ellisman, Mark H, McAdams, Harley H, Shapiro, Lucy
Format: Article
Language:English
Subjects:
Bacteria
Bacterial proteins
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Caulobacter
Caulobacter crescentus
Caulobacter crescentus - metabolism
Caulobacter crescentus - physiology
Cell Cycle
Cell Polarity
Cells
Centromere - metabolism
Chromosomes
Chromosomes, Bacterial - metabolism
DNA Replication
DNA, Bacterial - metabolism
Fundamental and applied biological sciences. Psychology
Microbiology
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Microscopy, Immunoelectron
Miscellaneous
Models, Biological
Models, Molecular
Morphology
Protein Multimerization
Ribonucleic acid
RNA
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Summary:The bacterium Caulobacter crescentus has morphologically and functionally distinct cell poles that undergo sequential changes during the cell cycle. We show that the PopZ oligomeric network forms polar ribosome exclusion zones that change function during cell cycle progression. The parS/ParB chromosomal centromere is tethered to PopZ at one pole prior to the initiation of DNA replication. During polar maturation, the PopZ-centromere tether is broken, and the PopZ zone at that pole then switches function to act as a recruitment factor for the ordered addition of multiple proteins that promote the transformation of the flagellated pole into a stalked pole. Stalked pole assembly, in turn, triggers the initiation of chromosome replication, which signals the formation of a new PopZ zone at the opposite cell pole, where it functions to anchor the newly duplicated centromere that has traversed the long axis of the cell. We propose that pole-specific control of PopZ function co-ordinates polar development and cell cycle progression by enabling independent assembly and tethering activities at the two cell poles.
ISSN:0950-382X
1365-2958
DOI:10.1111/j.1365-2958.2010.07088.x