A strategy for direct mapping and identification of mutations by whole-genome sequencing

Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome se...

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Bibliographic Details
Published in:Genetics (Austin) 2010-09, Vol.186 (1), p.427-430
Main Authors: Zuryn, Steven, Le Gras, Stéphanie, Jamet, Karine, Jarriault, Sophie
Format: Article
Language:English
Subjects:
Animal genetics
Animals
Biochemistry, Molecular Biology
Chromosome Mapping - methods
Development Biology
DNA Mutational Analysis - methods
Genes
Genetics
Genome, Bacterial - genetics
Genomics
Life Sciences
Mutagenesis
Mutation
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Summary:Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.110.119230