Gene expression profiles of human glioblastomas are associated with both tumor cytogenetics and histopathology

Despite the increasing knowledge about the genetic alterations and molecular pathways involved in gliomas, few studies have investigated the association between the gene expression profiles (GEP) and both cytogenetics and histopathology of gliomas. Here, we analyzed the GEP (U133Plus2.0 chip) of 40...

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Bibliographic Details
Published in:Neuro-oncology (Charlottesville, Va.) Va.), 2010-09, Vol.12 (9), p.991-1003
Main Authors: Vital, Ana Luísa, Tabernero, Maria Dolores, Castrillo, Abel, Rebelo, Olinda, Tão, Hermínio, Gomes, Fernando, Nieto, Ana Belen, Resende Oliveira, Catarina, Lopes, Maria Celeste, Orfao, Alberto
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Cluster Analysis
Cytogenetics
Female
Gene Expression Profiling
Glioblastoma - genetics
Glioblastoma - pathology
Humans
In Situ Hybridization, Fluorescence
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
Reviews
Young Adult
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Summary:Despite the increasing knowledge about the genetic alterations and molecular pathways involved in gliomas, few studies have investigated the association between the gene expression profiles (GEP) and both cytogenetics and histopathology of gliomas. Here, we analyzed the GEP (U133Plus2.0 chip) of 40 gliomas (35 astrocytic tumors, 3 oligodendrogliomas, and 2 mixed tumors) and their association with tumor cytogenetics and histopathology. Unsupervised and supervised analyses showed significantly different GEP in low- vs high-grade gliomas, the most discriminating genes including genes involved in the regulation of cell proliferation, apoptosis, DNA repair, and signal transduction. In turn, among glioblastoma multiforme (GBM), 3 subgroups of tumors were identified according to their GEP, which were closely associated with the cytogenetic profile of their ancestral tumor cell clones: (i) EGFR amplification, (ii) isolated trisomy 7, and (iii) more complex karyotypes. In summary, our results show a clear association between the GEP of gliomas and tumor histopathology; additionally, among grade IV astrocytoma, GEP are significantly associated with the cytogenetic profile of the ancestral tumor cell clone. Further studies in larger series of patients are necessary to confirm our observations.
ISSN:1522-8517
1523-5866
DOI:10.1093/neuonc/noq050