Advanced optical imaging in living embryos

Developmental biology investigations have evolved from static studies of embryo anatomy and into dynamic studies of the genetic and cellular mechanisms responsible for shaping the embryo anatomy. With the advancement of fluorescent protein fusions, the ability to visualize and comprehend how thousan...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2010-10, Vol.67 (20), p.3489-3497
Main Authors: Canaria, Christie A, Lansford, Rusty
Format: Article
Language:English
Subjects:
Anatomy
Anatomy & physiology
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cellular biology
Confocal
Embryo, Nonmammalian - anatomy & histology
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - embryology
embryogenesis
Embryology
Embryos
Fluorescence
Life Sciences
Medical imaging
Microscopy
Microscopy, Confocal - methods
Models, Animal
Molecular biology
Review
Time-lapse imaging
Two-photon
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Summary:Developmental biology investigations have evolved from static studies of embryo anatomy and into dynamic studies of the genetic and cellular mechanisms responsible for shaping the embryo anatomy. With the advancement of fluorescent protein fusions, the ability to visualize and comprehend how thousands to millions of cells interact with one another to form tissues and organs in three dimensions (xyz) over time (t) is just beginning to be realized and exploited. In this review, we explore recent advances utilizing confocal and multi-photon time-lapse microscopy to capture gene expression, cell behavior, and embryo development. From choosing the appropriate fluorophore, to labeling strategy, to experimental set-up, and data pipeline handling, this review covers the various aspects related to acquiring and analyzing multi-dimensional data sets. These innovative techniques in multi-dimensional imaging and analysis can be applied across a number of fields in time and space including protein dynamics to cell biology to morphogenesis.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-010-0440-5