Atomic-Level Mapping of Antibody Epitopes on a GPCR

Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPC...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2009-05, Vol.131 (20), p.6952-6954
Main Authors: Paes, Cheryl, Ingalls, Jada, Kampani, Karan, Sulli, Chidananda, Kakkar, Esha, Murray, Meredith, Kotelnikov, Valery, Greene, Tiffani A, Rucker, Joseph B, Doranz, Benjamin J
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Epitope Mapping - methods
Fluorescent Antibody Technique - methods
Immunodominant Epitopes - analysis
Models, Molecular
Mutagenesis
Polymerase Chain Reaction - methods
Receptors, CCR5 - genetics
Receptors, CCR5 - immunology
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Summary:Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja900186n