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Atomic-Level Mapping of Antibody Epitopes on a GPCR
Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPC...
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| Published in: | Journal of the American Chemical Society 2009-05, Vol.131 (20), p.6952-6954 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a469t-5645b746379ae1bca7e437d438bf6e8eda71ae8ba461dc9213401466a6d1e3223 |
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| cites | cdi_FETCH-LOGICAL-a469t-5645b746379ae1bca7e437d438bf6e8eda71ae8ba461dc9213401466a6d1e3223 |
| container_end_page | 6954 |
| container_issue | 20 |
| container_start_page | 6952 |
| container_title | Journal of the American Chemical Society |
| container_volume | 131 |
| creator | Paes, Cheryl Ingalls, Jada Kampani, Karan Sulli, Chidananda Kakkar, Esha Murray, Meredith Kotelnikov, Valery Greene, Tiffani A Rucker, Joseph B Doranz, Benjamin J |
| description | Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry. |
| doi_str_mv | 10.1021/ja900186n |
| format | article |
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Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja900186n</identifier><identifier>PMID: 19453194</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Epitope Mapping - methods ; Fluorescent Antibody Technique - methods ; Immunodominant Epitopes - analysis ; Models, Molecular ; Mutagenesis ; Polymerase Chain Reaction - methods ; Receptors, CCR5 - genetics ; Receptors, CCR5 - immunology</subject><ispartof>Journal of the American Chemical Society, 2009-05, Vol.131 (20), p.6952-6954</ispartof><rights>Copyright © 2009 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a469t-5645b746379ae1bca7e437d438bf6e8eda71ae8ba461dc9213401466a6d1e3223</citedby><cites>FETCH-LOGICAL-a469t-5645b746379ae1bca7e437d438bf6e8eda71ae8ba461dc9213401466a6d1e3223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19453194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paes, Cheryl</creatorcontrib><creatorcontrib>Ingalls, Jada</creatorcontrib><creatorcontrib>Kampani, Karan</creatorcontrib><creatorcontrib>Sulli, Chidananda</creatorcontrib><creatorcontrib>Kakkar, Esha</creatorcontrib><creatorcontrib>Murray, Meredith</creatorcontrib><creatorcontrib>Kotelnikov, Valery</creatorcontrib><creatorcontrib>Greene, Tiffani A</creatorcontrib><creatorcontrib>Rucker, Joseph B</creatorcontrib><creatorcontrib>Doranz, Benjamin J</creatorcontrib><title>Atomic-Level Mapping of Antibody Epitopes on a GPCR</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. 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| identifier | ISSN: 0002-7863 |
| ispartof | Journal of the American Chemical Society, 2009-05, Vol.131 (20), p.6952-6954 |
| issn | 0002-7863 1520-5126 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2943208 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Epitope Mapping - methods Fluorescent Antibody Technique - methods Immunodominant Epitopes - analysis Models, Molecular Mutagenesis Polymerase Chain Reaction - methods Receptors, CCR5 - genetics Receptors, CCR5 - immunology |
| title | Atomic-Level Mapping of Antibody Epitopes on a GPCR |
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