Endotoxin and cytokines increase hepatic messenger RNA levels and serum concentrations of apolipoprotein J (clusterin) in Syrian hamsters

Infection and inflammation induce alterations in hepatic synthesis and plasma concentrations of the acute phase proteins. Our results show that apolipoprotein (apo) J is a positive acute phase protein. Endotoxin (LPS), tumor necrosis factor (TNF), and interleukin (IL)-1 increased hepatic mRNA and se...

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Bibliographic Details
Published in:The Journal of clinical investigation 1994-09, Vol.94 (3), p.1304-1309
Main Authors: Hardardóttir, I, Kunitake, S T, Moser, A H, Doerrler, W T, Rapp, J H, Grünfeld, C, Feingold, K R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Cholesterol - blood
Clusterin
Cricetinae
Endotoxins - pharmacology
Gene Expression - drug effects
Glycoproteins - analysis
Glycoproteins - biosynthesis
Glycoproteins - blood
Humans
Interleukin-1 - pharmacology
Kinetics
Lipopolysaccharides - pharmacology
Lipoproteins - blood
Liver - drug effects
Liver - metabolism
Male
Mesocricetus
Molecular Chaperones
Molecular Sequence Data
Organ Specificity
Peptides - chemical synthesis
Peptides - immunology
Recombinant Proteins - pharmacology
RNA, Messenger - biosynthesis
Triglycerides - blood
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Infection and inflammation induce alterations in hepatic synthesis and plasma concentrations of the acute phase proteins. Our results show that apolipoprotein (apo) J is a positive acute phase protein. Endotoxin (LPS), tumor necrosis factor (TNF), and interleukin (IL)-1 increased hepatic mRNA and serum protein levels of apo J in Syrian hamsters. Hepatic apo J mRNA levels increased 10- to 15-fold with doses of LPS from 0.1 to 100 micrograms/100 g body weight within 4 h and were elevated for > or = 24 h. Serum apo J concentrations were significantly increased by 16 h and further elevated to 3.3 times that of control, 24 h after LPS administration. Serum apo J was associated with high density lipoprotein and increased fivefold in this fraction, after LPS administration. Hepatic apo J mRNA levels increased 3.5- and 4.6-fold, with TNF and IL-1, respectively, and 8.2-fold with a combination of TNF and IL-1. Serum apo J concentrations were increased 2.3-fold by TNF, 79% by IL-1, and 2.9-fold with a combination of TNF and IL-1. These results demonstrate that apo J is a positive acute phase protein.
ISSN:0021-9738
DOI:10.1172/JCI117449