Stabilization of glucose in blood samples: why it matters

[...] findings appear to reflect the fact that the major glycolytic enzyme targeted by fluoride, enolase (phosphopyruvate hydratase, E.C. 4.2.2.11), is located distally in the glycolytic pathway. [...] even in the presence of fluoride, glucose is phosphorylated by available ATP, and the glucose 6-ph...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2009-05, Vol.55 (5), p.850-852
Main Authors: Bruns, David E, Knowler, William C
Format: Article
Language:English
Subjects:
Blood Glucose - analysis
Blood Glucose - chemistry
Clinical medicine
Diabetes
Diabetes Mellitus - blood
Diabetic retinopathy
Fluorides
Glucose
Humans
Hyperglycemia
Laboratories
Plasma
Reproducibility of Results
Risk assessment
Specimen Handling - methods
Studies
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Summary:[...] findings appear to reflect the fact that the major glycolytic enzyme targeted by fluoride, enolase (phosphopyruvate hydratase, E.C. 4.2.2.11), is located distally in the glycolytic pathway. [...] even in the presence of fluoride, glucose is phosphorylated by available ATP, and the glucose 6-phosphate formed is further metabolized until equilibria are reached in reactions proximal to enolase in the glycolytic pathway (8). Diagnosis of Diabetes Cutpoints of plasma glucose concentrations are based on carefully designed studies, performed over many years, that provide the evidence base for the diagnosis of diabetes (2). Thus, a fasting plasma glucose concentration ≥7.0 mmol/L (≥126 mg/dL), rather than the various, higher cutpoints used in the past, is broadly accepted as diagnostic of diabetes (when observed on 2 or more occasions).
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2009.126037