Peroxiredoxin 2 is a novel autoantigen for anti‐endothelial cell antibodies in systemic vasculitis

Summary Anti‐endothelial cell antibodies (AECA) have been frequently detected in systemic vasculitis, which affects blood vessels of various sizes. To understand the pathogenic roles of AECA in systemic vasculitis, we attempted to identify target antigens for AECA comprehensively by a proteomic appr...

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Bibliographic Details
Published in:Clinical and experimental immunology 2010-09, Vol.161 (3), p.459-470
Main Authors: Karasawa, R., Kurokawa, M. S., Yudoh, K., Masuko, K., Ozaki, S., Kato, T.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Analytical, structural and metabolic biochemistry
anti‐endothelial cell antibodies
Autoantibodies - blood
Autoantibodies - immunology
Autoantibodies - pharmacology
Autoantigens - immunology
Biological and medical sciences
Blotting, Western
Cell Line
Cells, Cultured
Chemokines - metabolism
Cytokines - metabolism
Electrophoresis, Gel, Two-Dimensional
Endothelial Cells - drug effects
Endothelial Cells - immunology
Endothelial Cells - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Male
Medical sciences
Middle Aged
peroxiredoxin 2
Peroxiredoxins - immunology
Peroxiredoxins - metabolism
Proteins - immunology
Proteins - metabolism
proteomics
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
systemic vasculitis
Translational Studies
two‐dimensional electrophoresis
Vasculitis - blood
Vasculitis - immunology
Vasculitis - metabolism
Young Adult
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Summary:Summary Anti‐endothelial cell antibodies (AECA) have been frequently detected in systemic vasculitis, which affects blood vessels of various sizes. To understand the pathogenic roles of AECA in systemic vasculitis, we attempted to identify target antigens for AECA comprehensively by a proteomic approach. Proteins extracted from human umbilical vein endothelial cells (HUVEC) were separated by two‐dimensional electrophoresis, and Western blotting was subsequently conducted using sera from patients with systemic vasculitis. As a result, 53 autoantigenic protein spots for AECA were detected, nine of which were identified by mass spectrometry. One of the identified proteins was peroxiredoxin 2 (Prx2), an anti‐oxidant enzyme. Frequency of anti‐Prx2 autoantibodies, measured by enzyme‐linked immunosorbent assay (ELISA), was significantly higher in systemic vasculitis (60%) compared to those in collagen diseases without clinical vasculitis (7%, P 
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2010.04218.x