Cytochrome c oxidase loses catalytic activity and structural integrity during the aging process in Drosophila melanogaster

► Cytochrome c oxidase loses catalytic activity during the aging process. ► Abundance of seven nuclear-encoded subunits of cytochrome c oxidase decreased with age in Drosophila. ► Cytochrome c oxidase is specific intra-mitochondrial site of age-related deterioration. The hypothesis, that structural...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2010-10, Vol.401 (1), p.64-68
Main Authors: Ren, Jian-Ching, Rebrin, Igor, Klichko, Vladimir, Orr, William C., Sohal, Rajindar S.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AGING
Aging - metabolism
Animals
ANIONS
ANTIBODIES
Catalysis
Complex IV subunits
Cytochrome c oxidase
DNA
DROSOPHILA
Drosophila melanogaster - enzymology
Drosophila melanogaster - physiology
Electron transport chain
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - metabolism
HYDROGEN PEROXIDE
MITOCHONDRIA
OXIDASES
OXIDATION
Oxidative stress
PHYSIOLOGY
POLYPEPTIDES
Protein Subunits - chemistry
Protein Subunits - metabolism
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Summary:► Cytochrome c oxidase loses catalytic activity during the aging process. ► Abundance of seven nuclear-encoded subunits of cytochrome c oxidase decreased with age in Drosophila. ► Cytochrome c oxidase is specific intra-mitochondrial site of age-related deterioration. The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H 2O 2 generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, ranging from 11% to 40%, during aging. These and previous results suggest that CcO is a specific intra-mitochondrial site of age-related deterioration, which may have a broad impact on mitochondrial physiology.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.09.009