Spectrophotometric and reversed-phase high-performance liquid chromatographic method for the determination of doxophylline in pharmaceutical formulations

Two methods are described for determination of Doxophylline in a solid dosage form. The first method was based on ultraviolet (UV)-spectrophotometric determination of the drug. It involves absorbance measurement at 274 nm (λmax of Doxophylline) in 0.1 N hydrochloric acid. The calibration curve was l...

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Bibliographic Details
Published in:Journal of young pharmacists 2010-07, Vol.2 (3), p.289-296
Main Authors: Joshi, H, Patel, A, Captain, A
Format: Article
Language:English
Subjects:
Accuracy
Analytical chemistry
Chromatography
Dilution
Methods
Pharm Analysis
Standard deviation
Statistical analysis
Studies
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Summary:Two methods are described for determination of Doxophylline in a solid dosage form. The first method was based on ultraviolet (UV)-spectrophotometric determination of the drug. It involves absorbance measurement at 274 nm (λmax of Doxophylline) in 0.1 N hydrochloric acid. The calibration curve was linear, with the correlation coefficient between 0.99 and 1.0 over a concentration range of 0.20-30 mg/ml for the drug. The second method was based on high-performance liquid chromatography (HPLC) separation of the drug in reverse-phase mode using the Hypersil ODS C 18 column (250 X 4.6 mm, 5 mm). The mobile phase constituted of buffer acetonitrile (80:20) and pH adjusted to 3.0, with dilute orthophosphoric acid delivered at a flow rate 1.0 ml/min. Detection was performed at 210 nm. Separation was completed within 7 min. The calibration curve was linear, with the correlation coefficient between 0.99 and 1.0 over a concentration range of 0.165-30 mg/ml for the drug. The relative standard deviation was found to be
ISSN:0975-1483
0975-1505
DOI:10.4103/0975-1483.66791