Importance of Residue 13 and the C-Terminus for the Structure and Activity of the Antimicrobial Peptide Aurein 2.2

• Published in: Biophysical journal 2010-11, Vol.99 (9), p.2926-2935
• Main Authors: Cheng, John T.J., Hale, John D., Kindrachuk, Jason, Jessen, Havard, Elliott, Melissa, Hancock, Robert E.W., Straus, Suzana K.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino Acid Substitution; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial Cationic Peptides - chemistry; Antimicrobial Cationic Peptides - genetics; Antimicrobial Cationic Peptides - pharmacology; Biophysical Phenomena; Biophysics; Circular Dichroism; Hydrophobic and Hydrophilic Interactions; Lipid Bilayers - chemistry; Membrane; Membrane Potentials - drug effects; Membranes; Molecular Sequence Data; Molecular structure; Mutagenesis, Site-Directed; Mutation; NMR; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; Peptides; Protein Structure, Secondary; Spectrum analysis; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus epidermidis - drug effects; Staphylococcus infections; Structure-Activity Relationship; Studies
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2010.08.077

Previous studies on aurein 2.2 and 2.3 in DMPC/DMPG and POPC/POPG membranes have shown that bilayer thickness and phosphatidylglycerol content have a significant impact on the interaction of these peptides with membrane bilayers. Further examination with the DiSC 35 assay has indicated that aurein 2.2 induces greater membrane leakage than aurein 2.3 in Staphylococcus aureus C622. The only difference between these peptides is a Leu to Ile mutation at residue 13. To better understand the importance of this residue, the structure and activity of the L13A, L13F, and L13V mutants were investigated. In addition, we investigated a number of peptides with truncations at the C-terminus to determine whether the C-terminus, which contains residue 13, is crucial for antimicrobial activity. Solution circular dichroism results demonstrated that the L13F mutation and the truncation of the C-terminus by six residues resulted in decreased helical content, whereas the L13A or L13V mutation and the truncation of the C-terminus by three residues showed little to no effect on the structure. Oriented circular dichroism results demonstrated that only an extensive C-terminal truncation reduced the ability of the peptide to insert into lipid bilayers. 31P NMR spectroscopy showed that all peptides disorder the headgroups. The implications of these results in terms of antimicrobial activity and the ability of these peptides to induce leakage in S. aureus are discussed. The results suggest that the presence of the 13th residue in aurein 2.2 is important for structure and activity, but the exact nature of residue 13 is less important as long as it is a hydrophobic residue.