Transgenesis procedures in Xenopus

Stable integration of foreign DNA into the frog genome has been the purpose of several studies aimed at generating transgenic animals or producing mutations of endogenous genes. Inserting DNA into a host genome can be achieved in a number of ways. In Xenopus, different strategies have been developed...

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Bibliographic Details
Published in:Biology of the cell 2008-09, Vol.100 (9), p.503-529
Main Authors: Chesneau, Albert, Sachs, Laurent M., Chai, Norin, Chen, Yonglong, Du Pasquier, Louis, Loeber, Jana, Pollet, Nicolas, Reilly, Michael, Weeks, Daniel L., Bronchain, Odile J.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Anura
Gene Transfer Techniques
genetic modification
Genetic Techniques
Genetic Vectors
I-SceI meganuclease
Life Sciences
Neurons and Cognition
phi-C31 integrase
restriction-enzyme-mediated integration (REMI)
transgenesis workshop
Xenopus
Xenopus - genetics
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Summary:Stable integration of foreign DNA into the frog genome has been the purpose of several studies aimed at generating transgenic animals or producing mutations of endogenous genes. Inserting DNA into a host genome can be achieved in a number of ways. In Xenopus, different strategies have been developed which exhibit specific molecular and technical features. Although several of these technologies were also applied in various model organizms, the attributes of each method have rarely been experimentally compared. Investigators are thus confronted with a difficult choice to discriminate which method would be best suited for their applications. To gain better understanding, a transgenesis workshop was organized by the X‐omics consortium. Three procedures were assessed side‐by‐side, and the results obtained are used to illustrate this review. In addition, a number of reagents and tools have been set up for the purpose of gene expression and functional gene analyses. This not only improves the status of Xenopus as a powerful model for developmental studies, but also renders it suitable for sophisticated genetic approaches. Twenty years after the first reported transgenic Xenopus, we review the state of the art of transgenic research, focusing on the new perspectives in performing genetic studies in this species.
ISSN:0248-4900
1768-322X
DOI:10.1042/BC20070148