Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes t...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2010-11, Vol.132 (44), p.15522-15524
Main Authors: McGregor, Lynn M, Gorin, David J, Dumelin, Christoph E, Liu, David R
Format: Article
Language:English
Subjects:
Amplification
Binding
Binding Sites
Communication
Deoxyribonucleic acid
DNA - chemistry
Extensibility
Formations
Libraries
Ligands
Links
Polymerase Chain Reaction
Protein Binding
Small Molecule Libraries - chemistry
Solutions - chemistry
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Summary:Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja107677q