Genome-wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms

Our knowledge of Anopheles gambiae molecular biology has mainly been based on studies using inbred laboratory strains. Differences in the environmental exposure of these and natural field mosquitoes have inevitably led to physiological divergences. We have used global transcript abundance analyses t...

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Bibliographic Details
Published in:Insect molecular biology 2010-10, Vol.19 (5), p.695-705
Main Authors: Aguilar, R, Simard, F, Kamdem, C, Shields, T, Glass, G.E, Garver, L.S, Dimopoulos, G
Format: Article
Language:English
Subjects:
Animals
Anopheles - classification
Anopheles - genetics
Anopheles gambiae
Cameroon
Evolution, Molecular
Female
field mosquitoes
Gene Expression Profiling
Gene Frequency
Genetic Speciation
laboratory strains
M and S molecular forms
Microarray Analysis
Polymorphism, Genetic - physiology
transcriptomic divergence
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Summary:Our knowledge of Anopheles gambiae molecular biology has mainly been based on studies using inbred laboratory strains. Differences in the environmental exposure of these and natural field mosquitoes have inevitably led to physiological divergences. We have used global transcript abundance analyses to probe into this divergence, and identified transcript abundance patterns of genes that provide insight on specific adaptations of caged and field mosquitoes. We also compared the gene transcript abundance profiles of field mosquitoes belonging to the two morphologically indistinguishable but reproductively isolated sympatric molecular forms, M and S, from two different locations in the Yaoundé area of Cameroon. This analysis suggested that environmental exposure has a greater influence on the transcriptome than does the mosquito's molecular form-specific genetic background.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2010.01031.x