Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer

We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIP...

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Bibliographic Details
Published in:Nucleic acids research 2001-03, Vol.29 (6), p.E34-E34
Main Authors: Kurata, S, Kanagawa, T, Yamada, K, Torimura, M, Yokomaku, T, Kamagata, Y, Kurane, R
Format: Article
Language:English
Subjects:
Boron Compounds - chemistry
DNA - genetics
DNA - metabolism
DNA Primers - chemistry
DNA Primers - genetics
DNA Probes - chemistry
DNA Probes - genetics
Fluorescence
Globins - genetics
Humans
NAR Methods Online
Polymerase Chain Reaction - methods
RNA - genetics
RNA - metabolism
Spectrometry, Fluorescence
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Summary:We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIPY((R)) FL-modified cytosine at its 5'-end. When such a probe was hybridized with a target DNA, its fluorescence was quenched by the guanine in the target, complementary to the modified cytosine, and the quench rate was proportional to the amount of target DNA. This widely applicable technique will be used directly with larger samples or in conjunction with the polymerase chain reaction to quantify small DNA samples.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/29.6.e34