Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission

Septins are a family of GTP-binding proteins implicated in mammalian cell division. Most studies examining the role of septins in this process have treated the family as a whole, thus neglecting the possibility that individual members may have diverse functions. To address this, we individually depl...

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Bibliographic Details
Published in:The Journal of cell biology 2010-11, Vol.191 (4), p.741-749
Main Authors: Estey, Mathew P, Di Ciano-Oliveira, Caterina, Froese, Carol D, Bejide, Margaret T, Trimble, William S
Format: Article
Language:English
Subjects:
Abscission
Cell division
Cell Line
Cell lines
Cellular biology
Cytokinesis
Cytokinesis - physiology
Daughter cells
Epithelial cells
Gene expression
HEK293 cells
HeLa cells
Humans
Protein isoforms
Protein Isoforms - genetics
Protein Isoforms - metabolism
Proteins
Ribonucleic acid
RNA
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Septins - genetics
Septins - metabolism
Small interfering RNA
Time-Lapse Imaging
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Summary:Septins are a family of GTP-binding proteins implicated in mammalian cell division. Most studies examining the role of septins in this process have treated the family as a whole, thus neglecting the possibility that individual members may have diverse functions. To address this, we individually depleted each septin family member expressed in HeLa cells by siRNA and assayed for defects in cell division by immunofluorescence and time-lapse microscopy. Depletion of SEPT2, SEPT7, and SEPT11 causes defects in the early stages of cytokinesis, ultimately resulting in binucleation. In sharp contrast, SEPT9 is dispensable for the early stages of cell division, but is critical for the final separation of daughter cells. Rescue experiments indicate that SEPT9 isoforms containing the N-terminal region are sufficient to drive cytokinesis. We demonstrate that SEPT9 mediates the localization of the vesicle-tethering exocyst complex to the midbody, providing mechanistic insight into the role of SEPT9 during abscission.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.201006031