An Ancient P-Loop GTPase in Rice Is Regulated by a Higher Plant-specific Regulatory Protein

YchF is a subfamily of the Obg family in the TRAFAC class of P-loop GTPases. The wide distribution of YchF homologues in both eukarya and bacteria suggests that they are descendents of an ancient protein, yet their physiological roles remain unclear. Using the OsYchF1-OsGAP1 pair from rice as the pr...

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Bibliographic Details
Published in:The Journal of biological chemistry 2010-11, Vol.285 (48), p.37359-37369
Main Authors: Cheung, Ming-Yan, Xue, Yan, Zhou, Liang, Li, Man-Wah, Sun, Samuel Sai-Ming, Lam, Hon-Ming
Format: Article
Language:English
Subjects:
ATPases
Electron Microscopy (EM)
Evolution, Molecular
G Proteins
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
GTP Phosphohydrolases - chemistry
GTP Phosphohydrolases - genetics
GTP Phosphohydrolases - metabolism
GTPase-Activating Proteins - chemistry
GTPase-Activating Proteins - genetics
GTPase-Activating Proteins - metabolism
Molecular Sequence Data
Obg
Oryza - chemistry
Oryza - classification
Oryza - enzymology
Oryza - genetics
Phylogeny
Plant
Plant Biology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Protein Binding
Protein Structure, Tertiary
Rice
Signal Transduction
Species Specificity
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Summary:YchF is a subfamily of the Obg family in the TRAFAC class of P-loop GTPases. The wide distribution of YchF homologues in both eukarya and bacteria suggests that they are descendents of an ancient protein, yet their physiological roles remain unclear. Using the OsYchF1-OsGAP1 pair from rice as the prototype, we provide evidence for the regulation of GTPase/ATPase activities and RNA binding capacity of a plant YchF (OsYchF1) by its regulatory protein (OsGAP1). The effects of OsGAP1 on the subcellular localization/cycling and physiological functions of OsYchF1 are also discussed. The finding that OsYchF1 and OsGAP1 are involved in plant defense response might shed light on the functional roles of YchF homologues in plants. This work suggests that during evolution, an ancestral P-loop GTPase/ATPase may acquire new regulation and function(s) by the evolution of a lineage-specific regulatory protein.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M110.172080