Tissue-Specific Expression of Insulin-Like Growth Factor II mRNAs with Distinct 5′ Untranslated Regions

We have used RNA from human hypothalamus as template for the production of cDNAs encoding insulinlike growth factor II (IGF-II). The prohormone coding sequence of brain IGF-II RNA is identical to that found in liver; however, the 5′ untranslated sequence of the brain cDNA has no homology to the 5′ u...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (18), p.6330-6334
Main Authors: Irminger, Jean-Claude, Rosen, Kenneth M., Humbel, Rene E., Villa-Komaroff, Lydia
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Chromosome Mapping
Chromosomes, Human, Pair 11
Complementary DNA
DNA - genetics
Exons
Five prime untranslated regions
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetic engineering
Genetic technics
Humans
Hypothalamus
Hypothalamus - physiology
Insulin-Like Growth Factor II - genetics
Liver
Liver - physiology
Messenger RNA
Methods. Procedures. Technologies
Pheochromocytoma
Placenta
Placenta - physiology
Protein Biosynthesis
RNA
RNA, Messenger - genetics
Somatomedins - genetics
Tissue Distribution
Untranslated regions
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Summary:We have used RNA from human hypothalamus as template for the production of cDNAs encoding insulinlike growth factor II (IGF-II). The prohormone coding sequence of brain IGF-II RNA is identical to that found in liver; however, the 5′ untranslated sequence of the brain cDNA has no homology to the 5′ untranslated sequence of the previously reported liver cDNAs. By using hybridization to specific probes as well as a method based on the properties of RNase H, we found that the human IGF-II gene has at least three exons that encode alternative 5′ untranslated regions and that are expressed in a tissue-specific manner. A probe specific to the brain cDNA 5′ untranslated region hybridizes to a 6.0-kilobase transcript present in placenta, hypothalamus, adrenal gland, kidney, Wilms tumor, and a pheochromocytoma. The 5′ untranslated sequence of the brain cDNA does not hybridize to a 5.3-kilobase transcript found in liver or to a 5.0-kb transcript found in pheochromocytoma. By using RNase H to specifically fragment the IGF-II transcripts into 3′ and 5′ fragments, we found that the RNAs vary in size due to differences in the 5′ end but not the 3′ end.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.18.6330