Chronic exposure to the chemokine CCL3 enhances neuronal network activity in rat hippocampal cultures

Abstract We examined the effect of chronic CCL3 treatment on the properties of cultured rat hippocampal neurons to gain an understanding of the neuronal effects of CCL3 during neuroinflammatory disorders. Western blot assays showed that chronic exposure to CCL3 altered the level of specific neuronal...

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Bibliographic Details
Published in:Journal of neuroimmunology 2010-12, Vol.229 (1), p.73-80
Main Authors: Kuijpers, M, van Gassen, K.L.I, de Graan, P.N.E, Gruol, D
Format: Article
Language:English
Subjects:
2-Amino-5-phosphonovalerate - pharmacology
Allergy and Immunology
Animals
Animals, Newborn
Ca 2
Calcium - metabolism
Calcium Signaling - drug effects
Cell Survival - drug effects
Chemokine CCL3 - pharmacology
Drug Interactions
Epilepsy
Excitatory Amino Acid Antagonists - pharmacology
gamma-Aminobutyric Acid - pharmacology
Gene Expression Regulation - drug effects
Glial Fibrillary Acidic Protein - metabolism
Glutamate Decarboxylase - metabolism
Hippocampus - cytology
Hippocampus - drug effects
Nerve Net - drug effects
Neuroglia - drug effects
Neuroinflammation
Neurology
Neurons - drug effects
NMDA receptor
Organ Culture Techniques
Phosphopyruvate Hydratase - metabolism
Quinoxalines - pharmacology
Rats
Rats, Sprague-Dawley
Receptors, Glutamate - metabolism
Synaptic Potentials - drug effects
Synaptic transmission
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Summary:Abstract We examined the effect of chronic CCL3 treatment on the properties of cultured rat hippocampal neurons to gain an understanding of the neuronal effects of CCL3 during neuroinflammatory disorders. Western blot assays showed that chronic exposure to CCL3 altered the level of specific neuronal and glial proteins and that CCL3 had no effect on neuronal survival. CCL3 treatment also altered intracellular Ca2+ dynamics and increased Ca2+ levels in hippocampal neurons, measured by fura-2 imaging techniques. Additionally, chronic CCL3 increased NMDA-evoked Ca2+ signals in the hippocampal neurons and increased NMDA receptor levels. These CCL3-induced neuroadaptive changes could play an important role in the CNS dysfunction associated with CNS disorders with a neuroinflammatory component.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2010.07.004