The yeast vps class E mutants: the beginning of the molecular genetic analysis of multivesicular body biogenesis

In 1992, Raymond et al. published a compilation of the 41 yeast vacuolar protein sorting (vps) mutant groups and described a large class of mutants (class E vps mutants) that accumulated an exaggerated prevacuolar endosome-like compartment. Further analysis revealed that this "class E compartme...

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Bibliographic Details
Published in:Molecular biology of the cell 2010-12, Vol.21 (23), p.4057-4060
Main Authors: Coonrod, Emily M, Stevens, Tom H
Format: Article
Language:English
Subjects:
Endosomal Sorting Complexes Required for Transport - genetics
Endosomal Sorting Complexes Required for Transport - metabolism
Endosomes - metabolism
Golgi Apparatus - metabolism
History, 20th Century
Multivesicular Bodies - genetics
Multivesicular Bodies - metabolism
Mutation
Retrospective
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - ultrastructure
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Vacuoles - metabolism
Vacuoles - ultrastructure
Vesicular Transport Proteins - genetics
Vesicular Transport Proteins - metabolism
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Summary:In 1992, Raymond et al. published a compilation of the 41 yeast vacuolar protein sorting (vps) mutant groups and described a large class of mutants (class E vps mutants) that accumulated an exaggerated prevacuolar endosome-like compartment. Further analysis revealed that this "class E compartment" contained soluble vacuolar hydrolases, vacuolar membrane proteins, and Golgi membrane proteins unable to recycle back to the Golgi complex, yet these class E vps mutants had what seemed to be normal vacuoles. The 13 class E VPS genes were later shown to encode the proteins that make up the complexes required for formation of intralumenal vesicles in late endosomal compartments called multivesicular bodies, and for the sorting of ubiquitinated cargo proteins into these internal vesicles for eventual delivery to the vacuole or lysosome.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e09-07-0603