A Highly Selective Turn-On Colorimetric, Red Fluorescent Sensor for Detecting Mobile Zinc in Living Cells

We describe ZRL1, a turn-on colorimetric and red fluorescent zinc ion sensor. The Zn2+-promoted ring opening of the rhodamine spirolactam ring in ZRL1 evokes a 220-fold fluorescence turn-on response. In aqueous media, ZRL1 turn-on luminescence is highly selective for Zn2+ ions, with no significant r...

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Bibliographic Details
Published in:Inorganic chemistry 2010-12, Vol.49 (23), p.10753-10755
Main Authors: Du, Pingwu, Lippard, Stephen J
Format: Article
Language:English
Subjects:
Cell Survival
Colorimetry - methods
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Lactams - chemistry
Ligands
Models, Molecular
Rhodamines - chemistry
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Zinc - analysis
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Summary:We describe ZRL1, a turn-on colorimetric and red fluorescent zinc ion sensor. The Zn2+-promoted ring opening of the rhodamine spirolactam ring in ZRL1 evokes a 220-fold fluorescence turn-on response. In aqueous media, ZRL1 turn-on luminescence is highly selective for Zn2+ ions, with no significant response to other competitive cations, including Na+, K+, Ca2+, Mg2+, Mn2+, Fe2+, Co2+, Ni2+, Cu2+, Cd2+, or Hg2+. In addition to these characteristics, preliminary results indicate that ZRL1 can be delivered to living cells and can be used to monitor changes in intracellular Zn2+ levels.
ISSN:0020-1669
1520-510X
DOI:10.1021/ic101569a