Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis

The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene family encodes plant-specific transcription factors. In this report, the LBD gene DOWN IN DARK AND AUXIN1 (DDA1), which is closely related to LATERAL ORGAN BOUNDARIES (LOB) and ASYMMETRIC LEAVES2 (AS2), was characterized. DDA1 is expressed primarily in...

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Bibliographic Details
Published in:Journal of experimental botany 2011-01, Vol.62 (1), p.221-233
Main Authors: Mangeon, Amanda, Bell, Elizabeth M, Lin, Wan-ching, Jablonska, Barbara, Springer, Patricia S
Format: Article
Language:English
Subjects:
Arabidopsis - chemistry
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis - radiation effects
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Auxins
Biological and medical sciences
Ethanol
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Gene Expression Regulation, Plant - radiation effects
Genes
Hypocotyls
Indoleacetic Acids - metabolism
Light
Plant cells
Plant roots
Plants
Protein Structure, Tertiary
RESEARCH PAPER
Research Papers
Reverse transcriptase polymerase chain reaction
Seedlings
Signal Transduction - radiation effects
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene family encodes plant-specific transcription factors. In this report, the LBD gene DOWN IN DARK AND AUXIN1 (DDA1), which is closely related to LATERAL ORGAN BOUNDARIES (LOB) and ASYMMETRIC LEAVES2 (AS2), was characterized. DDA1 is expressed primarily in vascular tissues and its transcript levels were reduced by exposure to exogenous indole-3-acetic acid (IAA or auxin) and in response to dark exposure. Analysis of a T-DNA insertion line, dda1-1, in which the insertion resulted in misregulation of DDA1 transcripts in the presence of IAA and in the dark revealed possible functions in auxin response and photomorphogenesis. dda1-1 plants exhibited reduced sensitivity to auxin, produced fewer lateral roots, and displayed aberrant hypocotyl elongation in the dark. Phenotypes resulting from fusion of a transcriptional repression domain to DDA1 suggest that DDA1 may act as both a transcriptional activator and a transcriptional repressor depending on the context. These results indicate that DDA1 may function in both the auxin signalling and photomorphogenesis pathways.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erq259