A poly(A) Addition Site and a Downstream Termination Region are Required for Efficient Cessation of Transcription by RNA Polymerase II in the Mouse β maj-globin Gene

Sequence elements within the mouse β maj- globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chr...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-12, Vol.84 (23), p.8306-8310
Main Authors: Logan, John, Falck-Pedersen, Erik, Darnell, James E., Shenk, Thomas
Format: Article
Language:English
Subjects:
Adenoviruses
Animals
Biological and medical sciences
DNA
DNA Mutational Analysis
DNA probes
Fundamental and applied biological sciences. Psychology
Genes
Genes, Regulator
Globins - genetics
HeLa cells
Infections
Messenger RNA
Mice
Molecular and cellular biology
Molecular genetics
Poly A - genetics
RNA
RNA Polymerase II - genetics
RNA, Messenger - genetics
Terminator regions
Terminator Regions, Genetic
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Viruses
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Summary:Sequence elements within the mouse β maj- globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chromosome within its E1A transcription unit. Two β -globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of several single base pair substitutions into the AATAAA polyadenylylation motifs. These mutations inhibited both polyadenylylation and termination within the β -globin DNA segment. Therefore, poly(A) addition appears to be a prerequisite for efficient termination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.23.8306