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Detection of human cytomegalovirus in different histological types of gliomas

The association between human cytomegalovirus (HCMV) infection and glioblastoma has been a source of debate in recent years because of conflicting laboratory reports concerning the presence of the virus in glioma tissue. HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has...

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Published in:Acta neuropathologica 2008-07, Vol.116 (1), p.79-86
Main Authors: Scheurer, Michael E., Bondy, Melissa L., Aldape, Kenneth D., Albrecht, Thomas, El-Zein, Randa
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description The association between human cytomegalovirus (HCMV) infection and glioblastoma has been a source of debate in recent years because of conflicting laboratory reports concerning the presence of the virus in glioma tissue. HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has been shown to transform cells in vitro. Using sensitive immunohistochemical and in situ hybridization methods in 50 glioma samples, we detected HCMV antigen and DNA in 21/21 cases of glioblastoma, 9/12 cases of anaplastic gliomas and 14/17 cases of low-grade gliomas. Reactivity against the HCMV IE1 antigen (72 kDa) exhibited histology-specific patterns with more nuclear staining for anaplastic and low-grade gliomas, while GBMs showed nuclear and cytoplasmic staining that likely occurs with latent infection. Using IHC, the number of HCMV-positive cells in GBMs was 79% compared to 48% in lower grade tumors. Non-tumor areas of the tissue contained only four and 1% of HCMV-positive cells for GBMs and lower grade tumors, respectively. Hybridization to HCMV DNA in infected cells corresponded to patterns of immunoreactivity. Our findings support previous reports of the presence of HCMV infection in glioma tissues and advocate optimization of laboratory methods for the detection of active HCMV infections. This will allow for detection of low-level latent infections that may play an important role in the initiation and/or promotion of malignant gliomas.
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subjects Adult
Antibodies
Antigens
Brain cancer
Brain Neoplasms - virology
Cell cycle
Chicken pox
Cytomegalovirus
Cytomegalovirus Infections - complications
DNA, Viral - analysis
Epidemiology
Female
Glioma - virology
Herpes viruses
Herpesvirus
Human cytomegalovirus
Humans
Hybridization
Immediate-Early Proteins - analysis
Immunohistochemistry
In Situ Hybridization
Infections
Male
Medical research
Medicine
Medicine & Public Health
Middle Aged
Neurosciences
Original Paper
Pathogenesis
Pathology
Polymerase Chain Reaction
Tumors
title Detection of human cytomegalovirus in different histological types of gliomas
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