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Detection of human cytomegalovirus in different histological types of gliomas
The association between human cytomegalovirus (HCMV) infection and glioblastoma has been a source of debate in recent years because of conflicting laboratory reports concerning the presence of the virus in glioma tissue. HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has...
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Published in: | Acta neuropathologica 2008-07, Vol.116 (1), p.79-86 |
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description | The association between human cytomegalovirus (HCMV) infection and glioblastoma has been a source of debate in recent years because of conflicting laboratory reports concerning the presence of the virus in glioma tissue. HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has been shown to transform cells in vitro. Using sensitive immunohistochemical and in situ hybridization methods in 50 glioma samples, we detected HCMV antigen and DNA in 21/21 cases of glioblastoma, 9/12 cases of anaplastic gliomas and 14/17 cases of low-grade gliomas. Reactivity against the HCMV IE1 antigen (72 kDa) exhibited histology-specific patterns with more nuclear staining for anaplastic and low-grade gliomas, while GBMs showed nuclear and cytoplasmic staining that likely occurs with latent infection. Using IHC, the number of HCMV-positive cells in GBMs was 79% compared to 48% in lower grade tumors. Non-tumor areas of the tissue contained only four and 1% of HCMV-positive cells for GBMs and lower grade tumors, respectively. Hybridization to HCMV DNA in infected cells corresponded to patterns of immunoreactivity. Our findings support previous reports of the presence of HCMV infection in glioma tissues and advocate optimization of laboratory methods for the detection of active HCMV infections. This will allow for detection of low-level latent infections that may play an important role in the initiation and/or promotion of malignant gliomas. |
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HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has been shown to transform cells in vitro. Using sensitive immunohistochemical and in situ hybridization methods in 50 glioma samples, we detected HCMV antigen and DNA in 21/21 cases of glioblastoma, 9/12 cases of anaplastic gliomas and 14/17 cases of low-grade gliomas. Reactivity against the HCMV IE1 antigen (72 kDa) exhibited histology-specific patterns with more nuclear staining for anaplastic and low-grade gliomas, while GBMs showed nuclear and cytoplasmic staining that likely occurs with latent infection. Using IHC, the number of HCMV-positive cells in GBMs was 79% compared to 48% in lower grade tumors. Non-tumor areas of the tissue contained only four and 1% of HCMV-positive cells for GBMs and lower grade tumors, respectively. Hybridization to HCMV DNA in infected cells corresponded to patterns of immunoreactivity. Our findings support previous reports of the presence of HCMV infection in glioma tissues and advocate optimization of laboratory methods for the detection of active HCMV infections. 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HCMV is a ubiquitous herpesvirus that exhibits tropism for glial cells and has been shown to transform cells in vitro. Using sensitive immunohistochemical and in situ hybridization methods in 50 glioma samples, we detected HCMV antigen and DNA in 21/21 cases of glioblastoma, 9/12 cases of anaplastic gliomas and 14/17 cases of low-grade gliomas. Reactivity against the HCMV IE1 antigen (72 kDa) exhibited histology-specific patterns with more nuclear staining for anaplastic and low-grade gliomas, while GBMs showed nuclear and cytoplasmic staining that likely occurs with latent infection. Using IHC, the number of HCMV-positive cells in GBMs was 79% compared to 48% in lower grade tumors. Non-tumor areas of the tissue contained only four and 1% of HCMV-positive cells for GBMs and lower grade tumors, respectively. Hybridization to HCMV DNA in infected cells corresponded to patterns of immunoreactivity. Our findings support previous reports of the presence of HCMV infection in glioma tissues and advocate optimization of laboratory methods for the detection of active HCMV infections. This will allow for detection of low-level latent infections that may play an important role in the initiation and/or promotion of malignant gliomas.</description><subject>Adult</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Brain cancer</subject><subject>Brain Neoplasms - virology</subject><subject>Cell cycle</subject><subject>Chicken pox</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus Infections - complications</subject><subject>DNA, Viral - analysis</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Glioma - virology</subject><subject>Herpes viruses</subject><subject>Herpesvirus</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Immediate-Early Proteins - analysis</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Infections</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Pathogenesis</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Tumors</subject><issn>0001-6322</issn><issn>1432-0533</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v3CAQxVHVqtls-wF6qawecnMyGDBwiRRt_lXaKpf0jLANXiLbbMCOtN8-bLxq2khVTgjmN28e8xD6huEUA_CzCEAB5wAiB8Jkjj-gBaakyIER8hEtAFK1JEVxhI5jfEi3glP2GR1hQRgmJV-gX5dmNPXo_JB5m22mXg9ZvRt9b1rd-ScXppi5IWuctSaYYcw2Lo6-862rdZeNu62J-8a2c77X8Qv6ZHUXzdfDuUS_r6_uV7f5-u7m5-pindespGOOJWDdVAJXlaBMF4wIJoxkDSFV8tjY5A0slJRzyRkFS4ghJr1UUJfclmSJzmfd7VT1pqmTsaA7tQ2u12GnvHbq38rgNqr1T4q87IAngZODQPCPk4mj6l2sTdfpwfgpqlISEIKLd0EsmZSU0wT-eAM--CkMaQuqwFhIDhwShGeoDj7GYOwfyxjUPlI1R6pSpGofqcKp5_vff33tOGSYgGIGYioNrQmvk_-v-gzAo6wN</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Scheurer, Michael E.</creator><creator>Bondy, Melissa L.</creator><creator>Aldape, Kenneth D.</creator><creator>Albrecht, Thomas</creator><creator>El-Zein, Randa</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080701</creationdate><title>Detection of human cytomegalovirus in different histological types of gliomas</title><author>Scheurer, Michael E. ; 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subjects | Adult Antibodies Antigens Brain cancer Brain Neoplasms - virology Cell cycle Chicken pox Cytomegalovirus Cytomegalovirus Infections - complications DNA, Viral - analysis Epidemiology Female Glioma - virology Herpes viruses Herpesvirus Human cytomegalovirus Humans Hybridization Immediate-Early Proteins - analysis Immunohistochemistry In Situ Hybridization Infections Male Medical research Medicine Medicine & Public Health Middle Aged Neurosciences Original Paper Pathogenesis Pathology Polymerase Chain Reaction Tumors |
title | Detection of human cytomegalovirus in different histological types of gliomas |
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