Proliferative, structural and molecular features of the Mdx mouse prostate

• Published in: International journal of experimental pathology 2010-10, Vol.91 (5), p.408-419
• Main Authors: Pinto, Leslie C., Fávaro, Wagner J., Cagnon, Valéria H. A.
• Format: Article
• Language: English
• Subjects: Animals; Cell Division - physiology; Disease Models, Animal; Dystroglycans - metabolism; Estradiol - blood; immunohistochemistry; Male; mdx mice; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Microscopy, Electron, Transmission; Muscular Dystrophy, Animal - complications; Muscular Dystrophy, Animal - pathology; Muscular Dystrophy, Animal - physiopathology; Original; Prostate - pathology; Prostate - physiology; Prostate - ultrastructure; Prostatic Diseases - etiology; Prostatic Diseases - pathology; Prostatic Diseases - physiopathology; Testosterone - blood; ventral prostate; α-dystroglycan; β-dystroglycan
• ISSN: 0959-9673; 1365-2613
• DOI: 10.1111/j.1365-2613.2010.00722.x

Summary The prostate is fundamental to the male reproductive process, and the stroma–epithelium interaction has an important role in prostate maintenance. Studies suggest that dystroglycan (DG) plays a role in cancer development in various organs. Thus, the aims of this work were to characterize morphological and proliferative features of the prostatic stroma and epithelium of mdx mice; to verify the immunolocalization of the α and β DG, IGF‐1 and laminin α3 receptors; and to relate those structural and molecular events to prostate pathogenesis and to verify the viability of this experimental model in prostate studies. Thirty male mice (mdx and C57BL10/Uni) were divided into control and mdx groups. Samples from the ventral prostate were collected for immunological, Western Blotting, transmission electron microscopy and morphometric analyses. Oestradiol and testosterone measurements were verified. The results showed diminished testosterone and increased oestradiol levels in the mdx group. Atrophied cells and hypertrophied stroma were seen in the mdx mice. Weak α and β DG and laminin α3 immunolocalization was demonstrated in the mdx group. Intense insulin‐like growth factor receptor α‐1 (IGFRα‐1) localization was identified in the mdx animals. Thus, mdx animals showed changes in molecular and structural integrity and proliferation signals, leading to glandular homoeostasis imbalance, and compromise of prostate function. Also, the steroid hormone imbalance and the increased IGF‐1 receptor level detected in mdx mice could be considered as a crucial factor in the pathogenesis of prostatic disorders.